6GYB

Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri

6GYB の概要

• エントリーDOI: 10.2210/pdb6gyb/pdb
• EMDBエントリー: 0089
• 分子名称: VirB7, VirB9 protein, VirB10 protein (3 entities in total)
• 機能のキーワード: core complex, bacterial killing, protein transport, bacterial type iv secretion system, membrane protein
• 由来する生物種: Xanthomonas axonopodis pv. citri (strain 306); 詳細
• タンパク質・核酸の鎖数: 42
• 化学式量合計: 1225205.09

構造登録者

Sgro, G.G.,Costa, T.R.D.,Farah, C.S.,Waksman, G. (登録日: 2018-06-28, 公開日: 2018-10-24, 最終更新日: 2024-11-06)

主引用文献

Sgro, G.G.,Costa, T.R.D.,Cenens, W.,Souza, D.P.,Cassago, A.,Coutinho de Oliveira, L.,Salinas, R.K.,Portugal, R.V.,Farah, C.S.,Waksman, G.
Cryo-EM structure of the bacteria-killing type IV secretion system core complex from Xanthomonas citri.
Nat Microbiol, 3:1429-1440, 2018

PubMed Abstract: Type IV secretion (T4S) systems form the most common and versatile class of secretion systems in bacteria, capable of injecting both proteins and DNAs into host cells. T4S systems are typically composed of 12 components that form 2 major assemblies: the inner membrane complex embedded in the inner membrane and the core complex embedded in both the inner and outer membranes. Here we present the 3.3 Å-resolution cryo-electron microscopy model of the T4S system core complex from Xanthomonas citri, a phytopathogen that utilizes this system to kill bacterial competitors. An extensive mutational investigation was performed to probe the vast network of protein-protein interactions in this 1.13-MDa assembly. This structure expands our knowledge of the molecular details of T4S system organization, assembly and evolution.

PubMed: 30349081
DOI: 10.1038/s41564-018-0262-z

実験手法

ELECTRON MICROSCOPY (3.28 Å)