6GXJ

X-ray structure of DiRu-1-encapsulated Apoferritin

6GXJ の概要

• エントリーDOI: 10.2210/pdb6gxj/pdb
• 関連するPDBエントリー: 5ERK
• 分子名称: Ferritin light chain, CADMIUM ION, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: metal transport, metal-based compound encapsulation, ruthenium complex, ferritin nanocage
• 由来する生物種: Equus caballus (Horse)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21347.69

構造登録者

Pica, A.,Ferraro, G.,Merlino, A. (登録日: 2018-06-27, 公開日: 2019-02-06, 最終更新日: 2024-01-17)

主引用文献

Petruk, G.,Monti, D.M.,Ferraro, G.,Pica, A.,D'Elia, L.,Pane, F.,Amoresano, A.,Furrer, J.,Kowalski, K.,Merlino, A.
Encapsulation of the Dinuclear Trithiolato-Bridged Arene Ruthenium Complex Diruthenium-1 in an Apoferritin Nanocage: Structure and Cytotoxicity.
ChemMedChem, 14:594-602, 2019

PubMed Abstract: The effects of encapsulating the cytotoxic dinuclear trithiolato-bridged arene ruthenium complex [(η -p-MeC H iPr) Ru (μ -S-p-C H tBu) ]Cl (DiRu-1) within the apoferritin (AFt) nanocage were investigated. The DiRu-1-AFt nanocarrier was characterized by UV/Vis spectroscopy, ICP-MS, CD and X-ray crystallography. In contrast to previously reported Au- and Pt-based drug-loaded AFt carriers, we found no evidence of direct interactions between DiRu-1 and AFt. DiRu-1-AFt is cytotoxic toward immortalized murine BALB/c-3T3 fibroblasts transformed with SV40 virus (SVT2) and human epidermoid carcinoma A431 malignant cells, and exhibits moderate selectivity for these cancer cells over normal BALB/c-3T3 cells. DiRu-1-AFt triggers the production of reactive oxygen species, depolarization of mitochondrial membrane potential, and induces cell death via p53-mediated apoptosis. Comparison between our data and previous results suggests that the presence of specific interactions between a metal-based drug and AFt within the protein cage is not essential for drug encapsulation.

PubMed: 30674089
DOI: 10.1002/cmdc.201800805

実験手法

X-RAY DIFFRACTION (1.43 Å)