6GP0

Structure of mEos4b in the red fluorescent state

Summary for 6GP0

Entry DOI	10.2210/pdb6gp0/pdb
Related	6GOY 6GOZ 6GP1
Descriptor	Green to red photoconvertible GFP-like protein EosFP (2 entities in total)
Functional Keywords	fluorescent protein
Biological source	Lobophyllia hemprichii (stony corals)
Total number of polymer chains	1
Total formula weight	29548.29
Authors	De Zitter, E.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D. (deposition date: 2018-06-04, release date: 2019-05-22, Last modification date: 2024-01-17)
Primary citation	De Zitter, E.,Thedie, D.,Monkemoller, V.,Hugelier, S.,Beaudouin, J.,Adam, V.,Byrdin, M.,Van Meervelt, L.,Dedecker, P.,Bourgeois, D. Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM. Nat.Methods, 16:707-710, 2019 Cited by PubMed Abstract: Green-to-red photoconvertible fluorescent proteins repeatedly enter dark states, causing interrupted tracks in single-particle-tracking localization microscopy (sptPALM). We identified a long-lived dark state in photoconverted mEos4b that results from isomerization of the chromophore and efficiently absorbs cyan light. Addition of weak 488-nm light swiftly reverts this dark state to the fluorescent state. This strategy largely eliminates slow blinking and enables the recording of longer tracks in sptPALM with minimum effort. PubMed: 31285624 DOI: 10.1038/s41592-019-0462-3 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (1.5 Å)

Structure validation