6GIQ

Saccharomyces cerevisiae respiratory supercomplex III2IV

6GIQ の概要

• エントリーDOI: 10.2210/pdb6giq/pdb
• EMDBエントリー: 0004
• 分子名称: BJ4_G0001550.mRNA.1.CDS.1, BJ4_G0023510.mRNA.1.CDS.1, Cytochrome c oxidase subunit 1, ... (35 entities in total)
• 機能のキーワード: respiratory chain, supercomplex, bc1 complex, cytochrome c oxidase, electron transport
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 32
• 化学式量合計: 736651.78

構造登録者

Rathore, S.,Berndtsson, J.,Conrad, J.,Ott, M. (登録日: 2018-05-15, 公開日: 2019-01-02, 最終更新日: 2024-10-09)

主引用文献

Rathore, S.,Berndtsson, J.,Marin-Buera, L.,Conrad, J.,Carroni, M.,Brzezinski, P.,Ott, M.
Cryo-EM structure of the yeast respiratory supercomplex.
Nat. Struct. Mol. Biol., 26:50-57, 2019

PubMed Abstract: Respiratory chain complexes execute energy conversion by connecting electron transport with proton translocation over the inner mitochondrial membrane to fuel ATP synthesis. Notably, these complexes form multi-enzyme assemblies known as respiratory supercomplexes. Here we used single-particle cryo-EM to determine the structures of the yeast mitochondrial respiratory supercomplexes IIIIV and IIIIV, at 3.2-Å and 3.5-Å resolutions, respectively. We revealed the overall architecture of the supercomplex, which deviates from the previously determined assemblies in mammals; obtained a near-atomic structure of the yeast complex IV; and identified the protein-protein and protein-lipid interactions implicated in supercomplex formation. Take together, our results demonstrate convergent evolution of supercomplexes in mitochondria that, while building similar assemblies, results in substantially different arrangements and structural solutions to support energy conversion.

PubMed: 30598556
DOI: 10.1038/s41594-018-0169-7

実験手法

ELECTRON MICROSCOPY (3.23 Å)