6GIE

Crystal structure of the Acinetobacter baumannii outer membrane protein Omp33

Summary for 6GIE

• Entry DOI: 10.2210/pdb6gie/pdb
• Descriptor: 33-36 kDa outer membrane protein, (HYDROXYETHYLOXY)TRI(ETHYLOXY)OCTANE (3 entities in total)
• Functional Keywords: channel, membrane protein
• Biological source: Acinetobacter baumannii
• Total number of polymer chains: 1
• Total formula weight: 33563.16

Authors

Abellon-Ruiz, J.,Zahn, M.,Basle, A.,van den Berg, B. (deposition date: 2018-05-10, release date: 2018-09-19, Last modification date: 2024-05-01)

Primary citation

Abellon-Ruiz, J.,Zahn, M.,Basle, A.,van den Berg, B.
Crystal structure of the Acinetobacter baumannii outer membrane protein Omp33.
Acta Crystallogr D Struct Biol, 74:852-860, 2018

PubMed Abstract: Acinetobacter baumannii is becoming a major threat to human health due to its multidrug resistance. This is owing in a large part to the low permeability of its outer membrane (OM), which prevents high internal antibiotic concentrations and makes antibiotic-resistance mechanisms more effective. To exploit OM channels as potential delivery vehicles for future antibiotics, structural information is required. One abundant OM protein in A. baumannii is Omp33. This protein has been reported to be important for the in vivo fitness and virulence of A. baumannii, but its structure is not known. Here, the X-ray crystal structure of Omp33 is reported at a resolution of 2.1 Å. Omp33 has a 14-β-stranded barrel without stable extracellular loop constrictions. Instead, an extended and unusual periplasmic turn connecting β-strands 2 and 3 is present, which folds into the pore lumen and completely blocks the aqueous channel. The Omp33 structure helps in understanding how A. baumannii OM proteins contribute to the low permeability of the cell envelope of this bacterium and suggests that Omp33 might function as a gated channel.

PubMed: 30198896
DOI: 10.1107/S205979831800904X

Experimental method

X-RAY DIFFRACTION (2.1 Å)