6GHB

Crystal structure of Spx in complex with YjbH (oxidized)

6GHB の概要

• エントリーDOI: 10.2210/pdb6ghb/pdb
• 分子名称: Regulatory protein Spx, UPF0413 protein GK0824, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: regulatory protein spx adaptor protein yjbh, protein binding
• 由来する生物種: Bacillus subtilis (strain 168); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 98760.00

構造登録者

Awad, W.,Logan, D.T.,von Wachenfeldt, C. (登録日: 2018-05-06, 公開日: 2019-04-24, 最終更新日: 2024-11-20)

主引用文献

Awad, W.,Al-Eryani, Y.,Ekstrom, S.,Logan, D.T.,von Wachenfeldt, C.
Structural Basis for YjbH Adaptor-Mediated Recognition of Transcription Factor Spx.
Structure, 27:923-, 2019

PubMed Abstract: YjbH is a bacterial adaptor protein required for efficient proteolysis of the RNA polymerase-binding transcription factor Spx by the ClpXP protease. We report the structure of YjbH in complex with Spx. YjbH comprises a DsbA-like thioredoxin domain connected via a linker to a C-terminal domain reminiscent of the winged helix-turn-helix fold. The interaction between YjbH and Spx involves a large surface area. Binding to YjbH stabilizes the C-terminal ClpX recognition region of Spx. We show that mutation of critical YjbH contact residues abrogates Spx recognition. Small-angle X-ray scattering and hydrogen-deuterium exchange mass spectrometry analyses determined the existence of a stable heterodimeric complex in solution and provide evidence that binding of Spx to YjbH reduces the overall conformational flexibility of Spx. Our findings provide insights into the molecular basis for Spx recognition and suggest a model for how YjbH stabilizes Spx and displays the C terminus of Spx for engagement by ClpXP.

PubMed: 30982633
DOI: 10.1016/j.str.2019.03.009

実験手法

X-RAY DIFFRACTION (3.104 Å)