6GGR

Crystal structure of Salmonella zinc metalloprotease effector GtgA in complex with p65

6GGR の概要

• エントリーDOI: 10.2210/pdb6ggr/pdb
• 分子名称: Transcription factor p65, Bacteriophage virulence determinant, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: protease, metalloprotease, zinc, metal binding protein
• 由来する生物種: Mus musculus (House Mouse); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 43485.95

構造登録者

Jennings, E.,Esposito, D.,Rittinger, K.,Thurston, T. (登録日: 2018-05-03, 公開日: 2018-08-29, 最終更新日: 2024-01-17)

主引用文献

Jennings, E.,Esposito, D.,Rittinger, K.,Thurston, T.L.M.
Structure-function analyses of the bacterial zinc metalloprotease effector protein GtgA uncover key residues required for deactivating NF-kappa B.
J. Biol. Chem., 293:15316-15329, 2018

PubMed Abstract: The closely related type III secretion system zinc metalloprotease effector proteins GtgA, GogA, and PipA are translocated into host cells during infection. They then cleave nuclear factor κ-light-chain-enhancer of activated B cells (NF-κB) transcription factor subunits, dampening activation of the NF-κB signaling pathway and thereby suppressing host immune responses. We demonstrate here that GtgA, GogA, and PipA cleave a subset of NF-κB subunits, including p65, RelB, and cRel but not NF-κB1 and NF-κB2, whereas the functionally similar type III secretion system effector NleC of enteropathogenic and enterohemorrhagic cleaved all five NF-κB subunits. Mutational analysis of NF-κB subunits revealed that a single nonconserved residue in NF-κB1 and NF-κB2 that corresponds to the P1' residue Arg-41 in p65 prevents cleavage of these subunits by GtgA, GogA, and PipA, explaining the observed substrate specificity of these enzymes. Crystal structures of GtgA in its apo-form and in complex with the p65 N-terminal domain explained the importance of the P1' residue. Furthermore, the pattern of interactions suggested that GtgA recognizes NF-κB subunits by mimicking the shape and negative charge of the DNA phosphate backbone. Moreover, structure-based mutational analysis of GtgA uncovered amino acids that are required for the interaction of GtgA with p65, as well as those that are required for full activity of GtgA in suppressing NF-κB activation. This study therefore provides detailed and critical insight into the mechanism of substrate recognition by this family of proteins important for bacterial virulence.

PubMed: 30049795
DOI: 10.1074/jbc.RA118.004255

実験手法

X-RAY DIFFRACTION (2.097 Å)