6GEU

Crystal structure of the C230A mutant of human IBA57

6GEU の概要

• エントリーDOI: 10.2210/pdb6geu/pdb
• 関連するPDBエントリー: 5OLI 6ESR 6QE3 6QE4
• 分子名称: Putative transferase CAF17, mitochondrial (2 entities in total)
• 機能のキーワード: mitochondrial protein, fe-s protein biogenesis, infantile leukodystrophy, protein binding
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33767.78

構造登録者

Calderone, V.,Ciofi-Baffoni, S.,Gourdoupis, S.,Banci, L.,Nasta, V. (登録日: 2018-04-27, 公開日: 2019-04-03, 最終更新日: 2024-01-17)

主引用文献

Gourdoupis, S.,Nasta, V.,Calderone, V.,Ciofi-Baffoni, S.,Banci, L.
IBA57 Recruits ISCA2 to Form a [2Fe-2S] Cluster-Mediated Complex.
J.Am.Chem.Soc., 140:14401-14412, 2018

PubMed Abstract: The maturation of mitochondrial iron-sulfur proteins requires a complex protein machinery. Human IBA57 protein was proposed to act in a late phase of this machinery, along with GLRX5, ISCA1, and ISCA2. However, a molecular picture on how these proteins cooperate is not defined yet. We show here that IBA57 forms a heterodimeric complex with ISCA2 by bridging a [2Fe-2S] cluster, that [2Fe-2S] cluster binding is absolutely required to promote the complex formation, and that the cysteine of the conserved motif characterizing IBA57 protein family and the three conserved cysteines of the ISCA protein family act as cluster ligands. The [2Fe-2S] heterodimeric complex is the final product when IBA57 is either exposed to [2Fe-2S] ISCA2 or in the presence of [2Fe-2S] GLRX5 and apo ISCA2. We also find that the [2Fe-2S] ISCA2-IBA57 complex is resistant to highly oxidative environments and is capable of reactivating apo aconitase in vitro. Collectively, our data delinate a [2Fe-2S] cluster transfer pathway involving three partner proteins of the mitochondrial ISC machinery, that is, GLRX5, ISCA2 and IBA57, which leads to the formation of a [2Fe-2S] ISCA2-IBA57 complex.

PubMed: 30269484
DOI: 10.1021/jacs.8b09061

実験手法

X-RAY DIFFRACTION (1.55 Å)