6GEN

Chromatin remodeller-nucleosome complex at 4.5 A resolution.

6GEN の概要

• エントリーDOI: 10.2210/pdb6gen/pdb
• 関連するPDBエントリー: 6GEJ
• EMDBエントリー: 4395 4396
• 分子名称: Vacuolar protein sorting-associated protein 72, Vacuolar protein sorting-associated protein 71, RuvB-like protein 1, ... (16 entities in total)
• 機能のキーワード: chromatin, remodeller, atpase, histone, nuclear protein
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c); 詳細
• タンパク質・核酸の鎖数: 20
• 化学式量合計: 795586.86

構造登録者

Willhoft, O.,Chua, E.Y.D.,Wilkinson, M.,Wigley, D.B. (登録日: 2018-04-27, 公開日: 2018-10-17, 最終更新日: 2025-07-02)

主引用文献

Willhoft, O.,Ghoneim, M.,Lin, C.L.,Chua, E.Y.D.,Wilkinson, M.,Chaban, Y.,Ayala, R.,McCormack, E.A.,Ocloo, L.,Rueda, D.S.,Wigley, D.B.
Structure and dynamics of the yeast SWR1-nucleosome complex.
Science, 362:-, 2018

PubMed Abstract: The yeast SWR1 complex exchanges histone H2A in nucleosomes with Htz1 (H2A.Z in humans). The cryo-electron microscopy structure of the SWR1 complex bound to a nucleosome at 3.6-angstrom resolution reveals details of the intricate interactions between components of the SWR1 complex and its nucleosome substrate. Interactions between the Swr1 motor domains and the DNA wrap at superhelical location 2 distort the DNA, causing a bulge with concomitant translocation of the DNA by one base pair, coupled to conformational changes of the histone core. Furthermore, partial unwrapping of the DNA from the histone core takes place upon binding of nucleosomes to SWR1 complex. The unwrapping, as monitored by single-molecule data, is stabilized and has its dynamics altered by adenosine triphosphate binding but does not require hydrolysis.

PubMed: 30309918
DOI: 10.1126/science.aat7716

実験手法

ELECTRON MICROSCOPY (3.6 Å)