6G94

Structure of E. coli hydrogenase-1 C19G variant in complex with cytochrome b

Summary for 6G94

Entry DOI	10.2210/pdb6g94/pdb
Related	3UQY 3USC 3USE 4GD3
Descriptor	Hydrogenase-1 small chain, CHLORIDE ION, PROTOPORPHYRIN IX CONTAINING FE, ... (12 entities in total)
Functional Keywords	new [4fe-4s] cluster, oxidoreductase
Biological source	Escherichia coli K-12 More
Total number of polymer chains	10
Total formula weight	467579.08
Authors	Volbeda, A.,Fontecilla-Camps, J.C. (deposition date: 2018-04-10, release date: 2018-06-13, Last modification date: 2024-01-17)
Primary citation	Volbeda, A.,Mouesca, J.M.,Darnault, C.,Roessler, M.M.,Parkin, A.,Armstrong, F.A.,Fontecilla-Camps, J.C. X-ray structural, functional and computational studies of the O2-sensitive E. coli hydrogenase-1 C19G variant reveal an unusual [4Fe-4S] cluster. Chem. Commun. (Camb.), 54:7175-7178, 2018 Cited by PubMed Abstract: The crystal structure of the Escherichia coli O2-sensitive C19G [NiFe]-hydrogenase-1 variant shows that the mutation results in a novel FeS cluster, proximal to the Ni-Fe active site. While the proximal cluster of the native O2-tolerant enzyme can transfer two electrons to that site, EPR spectroscopy shows that the modified cluster can transfer only one electron, this shortfall coinciding with O2 sensitivity. Computational studies on electron transfer help to explain how the structural and redox properties of the novel FeS cluster modulate the observed phenotype. PubMed: 29888350 DOI: 10.1039/c8cc02896f PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.5 Å)

Structure validation