6G8I

14-3-3sigma in complex with a R124beta3R mutated YAP pS127 phosphopeptide

6G8I の概要

• エントリーDOI: 10.2210/pdb6g8i/pdb
• 分子名称: 14-3-3 protein sigma, ALA-HIS-SEP-SER-PRO-ALA-SER-LEU-GLN, CHLORIDE ION, ... (6 entities in total)
• 機能のキーワード: beta amino acid hippo pathway yap/taz, oncoprotein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 27822.83

構造登録者

Andrei, S.A.,Thijssen, V.,Brunsveld, L.,Ottmann, C.,Milroy, L.G. (登録日: 2018-04-09, 公開日: 2019-04-17, 最終更新日: 2024-11-20)

主引用文献

Andrei, S.A.,Thijssen, V.,Brunsveld, L.,Ottmann, C.,Milroy, L.G.
A study on the effect of synthetic alpha-to-beta3-amino acid mutations on the binding of phosphopeptides to 14-3-3 proteins.
Chem.Commun.(Camb.), 55:14809-14812, 2019

PubMed Abstract: Here we describe the synthesis of a series of α,β-phosphopeptides, based on the phosphoepitope site on YAP1 (yes-associated protein 1), and the biochemical, biophysical and structural characterization of their binding to 14-3-3 proteins. The impact of systematic mono- and di-substitution of α → β3 amino acid residues around the phosphoserine residue are discussed. Our results confirm the important role played by the +2 proline residue in the thermodynamics and structure of the phosphoepitope/14-3-3 interaction.

PubMed: 31763628
DOI: 10.1039/c9cc07982c

実験手法

X-RAY DIFFRACTION (1.6 Å)