6G6M
Crystal structure of the computationally designed Tako8 protein in P42212
Summary for 6G6M
| Entry DOI | 10.2210/pdb6g6m/pdb |
| Descriptor | Tako8, SULFATE ION (3 entities in total) |
| Functional Keywords | artificial protein, de novo protein, wd40 proteins |
| Biological source | synthetic construct |
| Total number of polymer chains | 1 |
| Total formula weight | 35408.48 |
| Authors | Noguchi, H.,Addy, C.,Simoncini, D.,Van Meervelt, L.,Schiex, T.,Zhang, K.Y.J.,Tame, J.R.H.,Voet, A.R.D. (deposition date: 2018-04-01, release date: 2018-11-28, Last modification date: 2024-05-08) |
| Primary citation | Noguchi, H.,Addy, C.,Simoncini, D.,Wouters, S.,Mylemans, B.,Van Meervelt, L.,Schiex, T.,Zhang, K.Y.J.,Tame, J.R.H.,Voet, A.R.D. Computational design of symmetrical eight-bladed beta-propeller proteins. IUCrJ, 6:46-55, 2019 Cited by PubMed Abstract: β-Propeller proteins form one of the largest families of protein structures, with a pseudo-symmetrical fold made up of subdomains called blades. They are not only abundant but are also involved in a wide variety of cellular processes, often by acting as a platform for the assembly of protein complexes. WD40 proteins are a subfamily of propeller proteins with no intrinsic enzymatic activity, but their stable, modular architecture and versatile surface have allowed evolution to adapt them to many vital roles. By computationally reverse-engineering the duplication, fusion and diversification events in the evolutionary history of a WD40 protein, a perfectly symmetrical homologue called Tako8 was made. If two or four blades of Tako8 are expressed as single polypeptides, they do not self-assemble to complete the eight-bladed architecture, which may be owing to the closely spaced negative charges inside the ring. A different computational approach was employed to redesign Tako8 to create Ika8, a fourfold-symmetrical protein in which neighbouring blades carry compensating charges. Ika2 and Ika4, carrying two or four blades per subunit, respectively, were found to assemble spontaneously into a complete eight-bladed ring in solution. These artificial eight-bladed rings may find applications in bionanotechnology and as models to study the folding and evolution of WD40 proteins. PubMed: 30713702DOI: 10.1107/S205225251801480X PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.7 Å) |
Structure validation
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