6FZO

SMURFP-Y56F mutant

6FZO の概要

• エントリーDOI: 10.2210/pdb6fzo/pdb
• 分子名称: Smurfp, SULFATE ION, 1,2-ETHANEDIOL, ... (5 entities in total)
• 機能のキーワード: smurfp, biliverdin, optoacustic, phycobiliprotein, fluorescent protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 64210.17

構造登録者

Janowski, R.,Fuenzalida-Wernera, J.P.,Mishra, K.,Vetschera, P.,Weidenfeld, I.,Richter, K.,Niessing, D.,Ntziachristos, V.,Stiel, A.C. (登録日: 2018-03-15, 公開日: 2018-10-17, 最終更新日: 2024-01-17)

主引用文献

Fuenzalida-Werner, J.P.,Janowski, R.,Mishra, K.,Weidenfeld, I.,Niessing, D.,Ntziachristos, V.,Stiel, A.C.
Crystal structure of a biliverdin-bound phycobiliprotein: Interdependence of oligomerization and chromophorylation.
J. Struct. Biol., 204:519-522, 2018

PubMed Abstract: Small, ultra-red fluorescence protein (smURFP) introduces the non-native biliverdin (BV) chromophore to phycobiliproteins (PBPs), allowing them to be used as transgenic labels for in vivo mammalian imaging. Presently, no structural information exists for PBPs bound to the non-native BV chromophore, which limits the further development of smURFP and related proteins as imaging labels or indicators. Here we describe the first crystal structure of a PBP bound to BV. The structures of smURFP-Y56R with BV and smURFP-Y56F without BV reveal unique oligomerization interfaces different from those in wild-type PBPs bound to native chromophores. Our structures suggest that the oligomerization interface affects the BV binding site, creating a link between oligomerization and chromophorylation that we confirmed through site-directed mutagenesis and that may help guide efforts to improve the notorious chromophorylation of smURFP and other PBPs engineered to bind BV.

PubMed: 30287387
DOI: 10.1016/j.jsb.2018.09.013

実験手法

X-RAY DIFFRACTION (2.3 Å)