6FZ0

Crystal structure of the metY SAM V riboswitch

6FZ0 の概要

• エントリーDOI: 10.2210/pdb6fz0/pdb
• 分子名称: metY SAM V (53-MER), SULFATE ION, SODIUM ION, ... (6 entities in total)
• 機能のキーワード: sam v riboswitch, pseudoknot, rna, gene regulation
• 由来する生物種: Candidatus Pelagibacter ubique HTCC1062
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 17690.89

構造登録者

Huang, L.,Lilley, D.M.J. (登録日: 2018-03-13, 公開日: 2018-07-04, 最終更新日: 2024-05-08)

主引用文献

Huang, L.,Lilley, D.M.J.
Structure and ligand binding of the SAM-V riboswitch.
Nucleic Acids Res., 46:6869-6879, 2018

PubMed Abstract: SAM-V is one of the class of riboswitches that bind S-adenosylmethione, regulating gene expression by controlling translation. We have solved the crystal structure of the metY SAM-V riboswitch bound to its SAM ligand at 2.5 Å resolution. The RNA folds as an H-type pseudoknot, with a major-groove triple helix in which resides the SAM ligand binding site. The bound SAM adopts an elongated conformation aligned with the axis of the triple helix, and is held at either end by hydrogen bonding to the adenine and the amino acid moieties. The central sulfonium cation makes electrostatic interactions with an U:A.U base triple, so conferring specificity. We propose a model in which SAM binding leads to association of the triplex third strand that stabilizes a short helix and occludes the ribosome binding site. Thus the new structure explains both ligand specificity and the mechanism of genetic control.

PubMed: 29931337
DOI: 10.1093/nar/gky520

実験手法

X-RAY DIFFRACTION (2.499 Å)