6FWS

Structure of DinG in complex with ssDNA and ADPBeF

6FWS の概要

• エントリーDOI: 10.2210/pdb6fws/pdb
• 分子名称: ATP-dependent DNA helicase DinG, DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), DNA (5'-D(*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3'), ... (8 entities in total)
• 機能のキーワード: atp, helicase, translocase, dna binding, dna binding protein
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 171142.91

構造登録者

Cheng, K.,Wigley, D. (登録日: 2018-03-07, 公開日: 2018-12-19, 最終更新日: 2024-05-08)

主引用文献

Cheng, K.,Wigley, D.B.
DNA translocation mechanism of an XPD family helicase.
Elife, 7:-, 2018

PubMed Abstract: The XPD family of helicases, that includes human disease-related FANCJ, DDX11 and RTEL1, are Superfamily two helicases that contain an iron-sulphur cluster domain, translocate on ssDNA in a 5'-3' direction and play important roles in genome stability. Consequently, mutations in several of these family members in eukaryotes cause human diseases. Family members in bacteria, such as the DinG helicase from , are also involved in DNA repair. Here we present crystal structures of complexes of DinG bound to single-stranded DNA (ssDNA) in the presence and absence of an ATP analogue (ADP•BeF), that suggest a mechanism for 5'-3' translocation along the ssDNA substrate. This proposed mechanism has implications for how those enzymes of the XPD family that recognise bulky DNA lesions might stall at these as the first step in initiating DNA repair. Biochemical data reveal roles for conserved residues that are mutated in human diseases.

PubMed: 30520735
DOI: 10.7554/eLife.42400

実験手法

X-RAY DIFFRACTION (2.5 Å)