6FUY

Crystal structure of human full-length vinculin-T12-A974K (residues 1-1066)

Replaces:  6FMM

Summary for 6FUY

• Entry DOI: 10.2210/pdb6fuy/pdb
• Descriptor: Vinculin, CALCIUM ION (3 entities in total)
• Functional Keywords: mechanosensitive self-inhibition adapter, cell adhesion
• Biological source: Homo sapiens (Human)
• Cellular location: Cell membrane ; Peripheral membrane protein ; Cytoplasmic side : P18206
• Total number of polymer chains: 1
• Total formula weight: 116699.31

Authors

Chorev, D.S.,Volberg, T.,Livne, A.,Eisenstein, M.,Martins, B.,Kam, Z.,Jockusch, B.M.,Medalia, O.,Sharon, M.,Geiger, B. (deposition date: 2018-02-28, release date: 2018-03-14, Last modification date: 2024-01-17)

Primary citation

Chorev, D.S.,Volberg, T.,Livne, A.,Eisenstein, M.,Martins, B.,Kam, Z.,Jockusch, B.M.,Medalia, O.,Sharon, M.,Geiger, B.
Conformational states during vinculin unlocking differentially regulate focal adhesion properties.
Sci Rep, 8:2693-2693, 2018

PubMed Abstract: Focal adhesions (FAs) are multi-protein complexes that connect the actin cytoskeleton to the extracellular matrix, via integrin receptors. The growth, stability and adhesive functionality of these structures are tightly regulated by mechanical stress, yet, despite the extensive characterization of the integrin adhesome, the detailed molecular mechanisms underlying FA mechanosensitivity are still unclear. Besides talin, another key candidate for regulating FA-associated mechanosensing, is vinculin, a prominent FA component, which possesses either closed ("auto-inhibited") or open ("active") conformation. A direct experimental demonstration, however, of the conformational transition between the two states is still absent. In this study, we combined multiple structural and biological approaches to probe the transition from the auto-inhibited to the active conformation, and determine its effects on FA structure and dynamics. We further show that the transition from a closed to an open conformation requires two sequential steps that can differentially regulate FA growth and stability.

PubMed: 29426917
DOI: 10.1038/s41598-018-21006-8

Experimental method

X-RAY DIFFRACTION (3 Å)