6FTI

Cryo-EM Structure of the Mammalian Oligosaccharyltransferase Bound to Sec61 and the Programmed 80S Ribosome

これはPDB形式変換不可エントリーです。

6FTI の概要

• エントリーDOI: 10.2210/pdb6fti/pdb
• EMDBエントリー: 4306 4307 4308 4309 4310 4311 4312 4313 4314 4315 4316 4317
• 分子名称: uL2, Ribosomal protein L11, eL13, ... (64 entities in total)
• 機能のキーワード: protein translocon of the endoplasmic reticulum, protein transport
• 由来する生物種: Oryctolagus cuniculus (Rabbit); 詳細
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 2289695.84

構造登録者

Braunger, K.,Becker, T.,Beckmann, R. (登録日: 2018-02-22, 公開日: 2018-03-21, 最終更新日: 2020-07-29)

主引用文献

Braunger, K.,Pfeffer, S.,Shrimal, S.,Gilmore, R.,Berninghausen, O.,Mandon, E.C.,Becker, T.,Forster, F.,Beckmann, R.
Structural basis for coupling protein transport and N-glycosylation at the mammalian endoplasmic reticulum.
Science, 360:215-219, 2018

PubMed Abstract: Protein synthesis, transport, and N-glycosylation are coupled at the mammalian endoplasmic reticulum by complex formation of a ribosome, the Sec61 protein-conducting channel, and oligosaccharyltransferase (OST). Here we used different cryo-electron microscopy approaches to determine structures of native and solubilized ribosome-Sec61-OST complexes. A molecular model for the catalytic OST subunit STT3A (staurosporine and temperature sensitive 3A) revealed how it is integrated into the OST and how STT3-paralog specificity for translocon-associated OST is achieved. The OST subunit DC2 was placed at the interface between Sec61 and STT3A, where it acts as a versatile module for recruitment of STT3A-containing OST to the ribosome-Sec61 complex. This detailed structural view on the molecular architecture of the cotranslational machinery for N-glycosylation provides the basis for a mechanistic understanding of glycoprotein biogenesis at the endoplasmic reticulum.

PubMed: 29519914
DOI: 10.1126/science.aar7899

実験手法

ELECTRON MICROSCOPY (4.2 Å)