6FT6

Structure of the Nop53 pre-60S particle bound to the exosome nuclear cofactors

これはPDB形式変換不可エントリーです。

6FT6 の概要

• エントリーDOI: 10.2210/pdb6ft6/pdb
• EMDBエントリー: 4301 4302
• 分子名称: 7S ribosomal RNA, Bud site selection protein 20, 60S ribosomal protein L11-A, ... (60 entities in total)
• 機能のキーワード: rna exosome, ribosome, pre-ribosome, mtr4, helicase, rna
• 由来する生物種: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast); 詳細
• タンパク質・核酸の鎖数: 57
• 化学式量合計: 2670685.70

構造登録者

Schuller, J.M.,Falk, S.,Conti, E. (登録日: 2018-02-20, 公開日: 2018-03-28, 最終更新日: 2025-07-09)

主引用文献

Schuller, J.M.,Falk, S.,Fromm, L.,Hurt, E.,Conti, E.
Structure of the nuclear exosome captured on a maturing preribosome.
Science, 360:219-222, 2018

PubMed Abstract: The RNA exosome complex processes and degrades a wide range of transcripts, including ribosomal RNAs (rRNAs). We used cryo-electron microscopy to visualize the yeast nuclear exosome holocomplex captured on a precursor large ribosomal subunit (pre-60) during 7-to-5.8 rRNA processing. The cofactors of the nuclear exosome are sandwiched between the ribonuclease core complex (Exo-10) and the remodeled "foot" structure of the pre-60 particle, which harbors the 5.8 rRNA precursor. The exosome-associated helicase Mtr4 recognizes the preribosomal substrate by docking to specific sites on the 25 rRNA, captures the 3' extension of the 5.8 rRNA, and channels it toward Exo-10. The structure elucidates how the exosome forms a structural and functional unit together with its massive pre-60 substrate to process rRNA during ribosome maturation.

PubMed: 29519915
DOI: 10.1126/science.aar5428

実験手法

ELECTRON MICROSCOPY (3.9 Å)