6FQ1

Crystal structure of the RRM12 domain of IMP3

6FQ1 の概要

• エントリーDOI: 10.2210/pdb6fq1/pdb
• 分子名称: Insulin-like growth factor 2 mRNA-binding protein 3, 1,2-ETHANEDIOL (3 entities in total)
• 機能のキーワード: rna recognition motif (rrm), imp3, igf2bp3, crystal structure., rna binding protein
• 由来する生物種: Homo sapiens (Human)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 38161.15

構造登録者

Jia, M.,Gut, H.,Chao, A.J. (登録日: 2018-02-12, 公開日: 2018-09-05, 最終更新日: 2024-01-17)

主引用文献

Jia, M.,Gut, H.,Chao, J.A.
Structural basis of IMP3 RRM12 recognition of RNA.
RNA, 24:1659-1666, 2018

PubMed Abstract: The IMP family of RNA binding proteins, also named as insulin-like growth factor 2 (IGF2) mRNA-binding proteins (IGF2BPs), are highly conserved RNA regulators that are involved in many RNA processing stages, including mRNA stability, localization, and translation. There are three paralogs in the IMP family, IMP1-3, in mammals that all adopt the same domain arrangement with two RNA recognition motifs (RRM) in the N terminus and four KH domains in the C terminus. Here, we report the structure and biochemical characterization of IMP3 RRM12 and its complex with two short RNAs. These structures show that both RRM domains of IMP3 adopt the canonical RRM topology with two α-helices packed on an anti-parallel four stranded β-sheet. The spatial orientation of RRM1 to RRM2 is unique compared with other known tandem RRM structures. In the IMP3 RRM12 complex with RNA, only RRM1 is involved in RNA binding and recognizes a dinucleotide sequence.

PubMed: 30135093
DOI: 10.1261/rna.065649.118

実験手法

X-RAY DIFFRACTION (1.31 Å)