6FPD

AB21 protein from Agaricus bisporus

6FPD の概要

• エントリーDOI: 10.2210/pdb6fpd/pdb
• 分子名称: Protein AB21, SODIUM ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: agaricus bisporus, tetrahelical bundle, toxin-like, unknown function
• 由来する生物種: Agaricus bisporus
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 22657.18

構造登録者

Houser, J.,Demo, G.,Komarek, J.,Wimmerova, M. (登録日: 2018-02-09, 公開日: 2018-05-16, 最終更新日: 2024-05-08)

主引用文献

Komarek, J.,Ivanov Kavkova, E.,Houser, J.,Horackova, A.,Zdanska, J.,Demo, G.,Wimmerova, M.
Structure and properties of AB21, a novel Agaricus bisporus protein with structural relation to bacterial pore-forming toxins.
Proteins, 86:897-911, 2018

PubMed Abstract: We report the characterization of the dimeric protein AB21 from Agaricus bisporus, one of the most commonly and widely consumed mushrooms in the world. The protein shares no significant sequence similarity with any protein of known function, and it is the first characterized member of its protein family. The coding sequence of the ab21 gene was determined and the protein was expressed in E. coli in a recombinant form. We demonstrated a high thermal and pH stability of AB21 and proved the weak affinity of the protein to divalent ions of some transition metals (nickel, zinc, cadmium, and cobalt). The reported crystallographic structure exhibits an interesting rod-like helical bundle fold with structural similarity to bacterial toxins of the ClyA superfamily. By immunostaining, we demonstrated an abundance of AB21 in the fruiting bodies of A. bisporus.

PubMed: 29722060
DOI: 10.1002/prot.25522

実験手法

X-RAY DIFFRACTION (2.5 Å)