6FLF

Deoxyguanylosuccinate synthase (DgsS) structure at 1.33 Angstrom resolution.

6FLF の概要

• エントリーDOI: 10.2210/pdb6flf/pdb
• 関連するPDBエントリー: 6FJR 6FKO
• 分子名称: Adenylosuccinate synthetase (2 entities in total)
• 機能のキーワード: 2, 6-diaminopurine, phage phivc8, synthetase, biosynthetic protein
• 由来する生物種: Vibrio phage phiVC8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 40393.00

構造登録者

Sleiman, D.,Loc'h, J.,Haouz, A.,Kaminski, P.A. (登録日: 2018-01-25, 公開日: 2019-06-12, 最終更新日: 2024-05-08)

主引用文献

Sleiman, D.,Garcia, P.S.,Lagune, M.,Loc'h, J.,Haouz, A.,Taib, N.,Rothlisberger, P.,Gribaldo, S.,Marliere, P.,Kaminski, P.A.
A third purine biosynthetic pathway encoded by aminoadenine-based viral DNA genomes.
Science, 372:516-520, 2021

PubMed Abstract: Cells have two purine pathways that synthesize adenine and guanine ribonucleotides from phosphoribose via inosylate. A chemical hybrid between adenine and guanine, 2-aminoadenine (Z), replaces adenine in the DNA of the cyanobacterial virus S-2L. We show that S-2L and phage PhiVC8 encode a third purine pathway catalyzed by PurZ, a distant paralog of succinoadenylate synthase (PurA), the enzyme condensing aspartate and inosylate in the adenine pathway. PurZ condenses aspartate with deoxyguanylate into dSMP (N6-succino-2-amino-2'-deoxyadenylate), which undergoes defumarylation and phosphorylation to give dZTP (2-amino-2'-deoxyadenosine-5'-triphosphate), a substrate for the phage DNA polymerase. Crystallography and phylogenetics analyses indicate a close relationship between phage PurZ and archaeal PurA enzymes. Our work elucidates the biocatalytic innovation that remodeled a DNA building block beyond canonical molecular biology.

PubMed: 33926955
DOI: 10.1126/science.abe6494

実験手法

X-RAY DIFFRACTION (1.33 Å)