6FKQ

THE CRYSTAL STRUCTURE OF A FRAGMENT OF NETRIN-1 IN COMPLEX WITH A FRAGMENT OF DRAXIN

6FKQ の概要

• エントリーDOI: 10.2210/pdb6fkq/pdb
• 関連するPDBエントリー: 4URT
• 関連するBIRD辞書のPRD_ID: PRD_900017
• 分子名称: Netrin-1, Draxin, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (7 entities in total)
• 機能のキーワード: axon guidance cue, netrin-1, draxin, commissural neuron, signaling protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 51699.61

構造登録者

Bhowmick, T.,Meijers, R. (登録日: 2018-01-24, 公開日: 2018-03-21, 最終更新日: 2024-01-17)

主引用文献

Liu, Y.,Bhowmick, T.,Liu, Y.,Gao, X.,Mertens, H.D.T.,Svergun, D.I.,Xiao, J.,Zhang, Y.,Wang, J.H.,Meijers, R.
Structural Basis for Draxin-Modulated Axon Guidance and Fasciculation by Netrin-1 through DCC.
Neuron, 97:1261-1267.e4, 2018

PubMed Abstract: Axon guidance involves the spatiotemporal interplay between guidance cues and membrane-bound cell-surface receptors, present on the growth cone of the axon. Netrin-1 is a prototypical guidance cue that binds to deleted in colorectal cancer (DCC), and it has been proposed that the guidance cue Draxin modulates this interaction. Here, we present structural snapshots of Draxin/DCC and Draxin/Netrin-1 complexes, revealing a triangular relationship that affects Netrin-mediated haptotaxis and fasciculation. Draxin interacts with DCC through the N-terminal four immunoglobulin domains, and Netrin-1 through the EGF-3 domain, in the same region where DCC binds. Netrin-1 and DCC bind to adjacent sites on Draxin, which appears to capture Netrin-1 and tether it to the DCC receptor. We propose the conformational flexibility of the single-pass membrane receptor DCC is used to promote fasciculation and regulate axon guidance through concerted Netrin-1/Draxin binding. VIDEO ABSTRACT.

PubMed: 29503192
DOI: 10.1016/j.neuron.2018.02.010

実験手法

X-RAY DIFFRACTION (3.07 Å)