6FIG

Crystal structure of the ANX1 ectodomain from Arabidopsis thaliana

6FIG の概要

• エントリーDOI: 10.2210/pdb6fig/pdb
• 分子名称: Receptor-like protein kinase ANXUR1, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-[alpha-L-fucopyranose-(1-6)]2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: receptor kinase, malectin-like domain, membrane protein, arabidopsis thaliana, plant protein
• 由来する生物種: Arabidopsis thaliana (thale cress)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 95717.61

構造登録者

Santiago, J. (登録日: 2018-01-18, 公開日: 2018-07-11, 最終更新日: 2024-11-13)

主引用文献

Moussu, S.,Augustin, S.,Roman, A.O.,Broyart, C.,Santiago, J.
Crystal structures of two tandem malectin-like receptor kinases involved in plant reproduction.
Acta Crystallogr D Struct Biol, 74:671-680, 2018

PubMed Abstract: Complex cell-to-cell communication between the male pollen tube and the female reproductive organs is required for plant fertilization. A family of Catharanthus roseus receptor kinase 1-like (CrRLK1L) membrane receptors has been genetically implicated in this process. Here, crystal structures of the CrRLK1Ls ANXUR1 and ANXUR2 are reported at 1.48 and 1.1 Å resolution, respectively. The structures reveal a novel arrangement of two malectin-like domains connected by a short β-hairpin linker and stabilized by calcium ions. The canonical carbohydrate-interaction surfaces of related animal and bacterial carbohydrate-binding modules are not conserved in plant CrRLK1Ls. In line with this, the binding of chemically diverse oligosaccharides to ANXUR1 and HERCULES1 could not be detected. Instead, CrRLK1Ls have evolved a protein-protein interface between their malectin domains which forms a deep cleft lined by highly conserved aromatic and polar residues. Analysis of the glycosylation patterns of different CrRLK1Ls and their oligomeric states suggests that this cleft could resemble a binding site for a ligand required for receptor activation of CrRLK1Ls.

PubMed: 29968676
DOI: 10.1107/S205979831800774X

実験手法

X-RAY DIFFRACTION (1.48 Å)