6FHV

Crystal structure of Penicillium oxalicum Glucoamylase

6FHV の概要

• エントリーDOI: 10.2210/pdb6fhv/pdb
• 分子名称: Glucoamylase, 2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, beta-D-mannopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose-(1-4)-2-acetamido-2-deoxy-beta-D-glucopyranose, ... (8 entities in total)
• 機能のキーワード: glycosylation, starch degradation, glycoside hydrolase, hydrolase
• 由来する生物種: Penicillium oxalicum (strain 114-2 / CGMCC 5302) (Penicillium decumbens)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 67263.63

構造登録者

Roth, C.,Moroz, O.V.,Ariza, A.,Friis, E.P.,Davies, G.J.,Wilson, K.S. (登録日: 2018-01-15, 公開日: 2018-05-09, 最終更新日: 2024-10-23)

主引用文献

Roth, C.,Moroz, O.V.,Ariza, A.,Skov, L.K.,Ayabe, K.,Davies, G.J.,Wilson, K.S.
Structural insight into industrially relevant glucoamylases: flexible positions of starch-binding domains.
Acta Crystallogr D Struct Biol, 74:463-470, 2018

PubMed Abstract: Glucoamylases are one of the most important classes of enzymes in the industrial degradation of starch biomass. They consist of a catalytic domain and a carbohydrate-binding domain (CBM), with the latter being important for the interaction with the polymeric substrate. Whereas the catalytic mechanisms and structures of the individual domains are well known, the spatial arrangement of the domains with respect to each other and its influence on activity are not fully understood. Here, the structures of three industrially used fungal glucoamylases, two of which are full length, have been crystallized and determined. It is shown for the first time that the relative orientation between the CBM and the catalytic domain is flexible, as they can adopt different orientations independently of ligand binding, suggesting a role as an anchor to increase the contact time and the relative concentration of substrate near the active site. The flexibility in the orientations of the two domains presented a considerable challenge for the crystallization of the enzymes.

PubMed: 29717717
DOI: 10.1107/S2059798318004989

実験手法

X-RAY DIFFRACTION (2 Å)