6FGB

Human FcRn extra-cellular domain complexed with Fab fragment of Rozanolixizumab

6FGB の概要

• エントリーDOI: 10.2210/pdb6fgb/pdb
• 分子名称: IgG receptor FcRn large subunit p51, Beta-2-microglobulin, 1519.g57- Light chain, ... (7 entities in total)
• 機能のキーワード: fcrn, rozanolixizumab, ecd, fab, immune system
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 97353.17

構造登録者

Sarkar, K.,Ceska, T.,Meier, C. (登録日: 2018-01-10, 公開日: 2018-08-29, 最終更新日: 2024-11-06)

主引用文献

Smith, B.,Kiessling, A.,Lledo-Garcia, R.,Dixon, K.L.,Christodoulou, L.,Catley, M.C.,Atherfold, P.,D'Hooghe, L.E.,Finney, H.,Greenslade, K.,Hailu, H.,Kevorkian, L.,Lightwood, D.,Meier, C.,Munro, R.,Qureshi, O.,Sarkar, K.,Shaw, S.P.,Tewari, R.,Turner, A.,Tyson, K.,West, S.,Shaw, S.,Brennan, F.R.
Generation and characterization of a high affinity anti-human FcRn antibody, rozanolixizumab, and the effects of different molecular formats on the reduction of plasma IgG concentration.
MAbs, 10:1111-1130, 2018

PubMed Abstract: Rozanolixizumab (UCB7665), a humanized high-affinity anti-human neonatal Fc receptor (FcRn) monoclonal antibody (IgG4P), has been developed to reduce pathogenic IgG in autoimmune and alloimmune diseases. We document the antibody isolation and compare rozanolixizumab with the same variable region expressed in various mono-, bi- and trivalent formats. We report activity data for rozanolixizumab and the different molecular formats in human cells, FcRn-transgenic mice, and cynomolgus monkeys. Rozanolixizumab, considered the most effective molecular format, dose-dependently and selectively reduced plasma IgG concentrations in an FcRn-transgenic mouse model (no effect on albumin). Intravenous (IV) rozanolixizumab dosing in cynomolgus monkeys demonstrated non-linear pharmacokinetics indicative of target-mediated drug disposition; single IV rozanolixizumab doses (30 mg/kg) in cynomolgus monkeys reduced plasma IgG concentration by 69% by Day 7 post-administration. Daily IV administration of rozanolixizumab (initial 30 mg/kg loading dose; 5 mg/kg daily thereafter) reduced plasma IgG concentrations in all cynomolgus monkeys, with low concentrations maintained throughout the treatment period (42 days). In a 13-week toxicology study in cynomolgus monkeys, supra-pharmacological subcutaneous and IV doses of rozanolixizumab (≤ 150 mg/kg every 3 days) were well tolerated, inducing sustained (but reversible) reductions in IgG concentrations by up to 85%, with no adverse events observed. We have demonstrated accelerated natural catabolism of IgG through inhibition of IgG:FcRn interactions in mice and cynomolgus monkeys. Inhibition of FcRn with rozanolixizumab may provide a novel therapeutic approach to reduce pathogenic IgG in human autoimmune disease. Rozanolixizumab is being investigated in patients with immune thrombocytopenia (NCT02718716) and myasthenia gravis (NCT03052751).

PubMed: 30130439
DOI: 10.1080/19420862.2018.1505464

実験手法

X-RAY DIFFRACTION (2.9 Å)