6FF9

Mutant R280K of human P53

6FF9 の概要

• エントリーDOI: 10.2210/pdb6ff9/pdb
• 分子名称: Cellular tumor antigen p53, ZINC ION (3 entities in total)
• 機能のキーワード: p53, mutant, dna binding, anticancer therapy, dna binding protein
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Cytoplasm. Isoform 1: Nucleus. Isoform 2: Nucleus. Isoform 3: Nucleus. Isoform 4: Nucleus. Isoform 7: Nucleus. Isoform 8: Nucleus. Isoform 9: Cytoplasm: P04637
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 87236.56

構造登録者

Trovao, F.G.,Gomes, A.S.,Pinheiro, B.,Carvalho, A.L.,Romao, M.J. (登録日: 2018-01-04, 公開日: 2018-04-25, 最終更新日: 2024-01-17)

主引用文献

Gomes, A.S.,Trovao, F.,Andrade Pinheiro, B.,Freire, F.,Gomes, S.,Oliveira, C.,Domingues, L.,Romao, M.J.,Saraiva, L.,Carvalho, A.L.
The Crystal Structure of the R280K Mutant of Human p53 Explains the Loss of DNA Binding.
Int J Mol Sci, 19:-, 2018

PubMed Abstract: The p53 tumor suppressor is widely found to be mutated in human cancer. This protein is regarded as a molecular hub regulating different cell responses, namely cell death. Compelling data have demonstrated that the impairment of p53 activity correlates with tumor development and maintenance. For these reasons, the reactivation of p53 function is regarded as a promising strategy to halt cancer. In the present work, the recombinant mutant p53R280K DNA binding domain (DBD) was produced for the first time, and its crystal structure was determined in the absence of DNA to a resolution of 2.0 Å. The solved structure contains four molecules in the asymmetric unit, four zinc(II) ions, and 336 water molecules. The structure was compared with the wild-type p53 DBD structure, isolated and in complex with DNA. These comparisons contributed to a deeper understanding of the mutant p53R280K structure, as well as the loss of DNA binding related to halted transcriptional activity. The structural information derived may also contribute to the rational design of mutant p53 reactivating molecules with potential application in cancer treatment.

PubMed: 29652801
DOI: 10.3390/ijms19041184

実験手法

X-RAY DIFFRACTION (2 Å)