6FB5
Crystal Structure of a Tailored I-CreI Homing Endonuclease Protein (3115 variant) in complex with an altered version of its target DNA (Haemoglobin beta subunit gene) at 5NNN region in the presence of Magnesium
Summary for 6FB5
Entry DOI | 10.2210/pdb6fb5/pdb |
Descriptor | I-CreI monomer A, I-CreI monomer B, DNA (5'-D(*TP*CP*AP*GP*AP*CP*TP*TP*GP*TP*CP*CP*AP*CP*AP*GP*GP*AP*GP*TP*CP*AP*GP*A)-3'), ... (8 entities in total) |
Functional Keywords | chlamydomonas reinhardtii, dna binding protein |
Biological source | Chlamydomonas reinhardtii More |
Total number of polymer chains | 4 |
Total formula weight | 50083.25 |
Authors | Molina, R.,Prieto, J. (deposition date: 2017-12-18, release date: 2018-07-18, Last modification date: 2024-01-17) |
Primary citation | Prieto, J.,Redondo, P.,Lopez-Mendez, B.,D'Abramo, M.,Merino, N.,Blanco, F.J.,Duchateau, P.,Montoya, G.,Molina, R. Understanding the indirect DNA read-out specificity of I-CreI Meganuclease. Sci Rep, 8:10286-10286, 2018 Cited by PubMed: 29980759DOI: 10.1038/s41598-018-28599-0 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (2.2 Å) |
Structure validation
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