6F9N

CRYSTAL STRUCTURE OF THE HUMAN CPSF160-WDR33 COMPLEX

6F9N の概要

• エントリーDOI: 10.2210/pdb6f9n/pdb
• 分子名称: Cleavage and polyadenylation specificity factor subunit 1, pre-mRNA 3' end processing protein WDR33 (3 entities in total)
• 機能のキーワード: polyadenylation, mrna, beta propeller, cpsf, 3' end processing, rna binding protein
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Nucleus, nucleoplasm: Q10570; Nucleus : Q9C0J8
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 204400.42

構造登録者

Clerici, M.,Jinek, M. (登録日: 2017-12-14, 公開日: 2018-01-03, 最終更新日: 2024-01-17)

主引用文献

Clerici, M.,Faini, M.,Aebersold, R.,Jinek, M.
Structural insights into the assembly and polyA signal recognition mechanism of the human CPSF complex.
Elife, 6:-, 2017

PubMed Abstract: 3' polyadenylation is a key step in eukaryotic mRNA biogenesis. In mammalian cells, this process is dependent on the recognition of the hexanucleotide AAUAAA motif in the pre-mRNA polyadenylation signal by the cleavage and polyadenylation specificity factor (CPSF) complex. A core CPSF complex comprising CPSF160, WDR33, CPSF30 and Fip1 is sufficient for AAUAAA motif recognition, yet the molecular interactions underpinning its assembly and mechanism of PAS recognition are not understood. Based on cross-linking-coupled mass spectrometry, crystal structure of the CPSF160-WDR33 subcomplex and biochemical assays, we define the molecular architecture of the core human CPSF complex, identifying specific domains involved in inter-subunit interactions. In addition to zinc finger domains in CPSF30, we identify using quantitative RNA-binding assays an N-terminal lysine/arginine-rich motif in WDR33 as a critical determinant of specific AAUAAA motif recognition. Together, these results shed light on the function of CPSF in mediating PAS-dependent RNA cleavage and polyadenylation.

PubMed: 29274231
DOI: 10.7554/eLife.33111

実験手法

X-RAY DIFFRACTION (2.5 Å)