6F6P

Crystal structure of tetrameric human Rabin8 GEF domain

「5OAG」から置き換えられました

6F6P の概要

• エントリーDOI: 10.2210/pdb6f6p/pdb
• 分子名称: Rab-3A-interacting protein (3 entities in total)
• 機能のキーワード: rabin8, gef, guanine nucleotide exchange factor, tetramer, rab3i, hydrolase
• 由来する生物種: Homo sapiens (Human); 詳細
• 細胞内の位置: Cytoplasm: Q96QF0 Q96QF0
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 47994.94

構造登録者

Lorentzen, E.,Vetter, M. (登録日: 2017-12-06, 公開日: 2017-12-13, 最終更新日: 2024-05-08)

主引用文献

Vetter, M.,Boegholm, N.,Christensen, A.,Bhogaraju, S.,Andersen, M.B.,Lorentzen, A.,Lorentzen, E.
Crystal structure of tetrameric human Rabin8 GEF domain.
Proteins, 86:405-413, 2018

PubMed Abstract: Rab GTPases and their effectors, activators and guanine nucleotide exchange factors (GEFs) are essential for vesicular transport. Rab8 and its GEF Rabin8 function in formation of the cilium organelle important for developmental signaling and sensory reception. Here, we show by size exclusion chromatography and analytical ultracentrifugation that Rabin8 exists in equilibrium between dimers and tetramers. The crystal structure of tetrameric Rabin8 GEF domain reveals an occluded Rab8 binding site suggesting that this oligomer is enzymatically inactive, a notion we verify experimentally using Rabin8/Rab8 GEF assays. We outline a procedure for the purification of active dimeric Rabin8 GEF-domain for in vitro activity assays.

PubMed: 29318657
DOI: 10.1002/prot.25455

実験手法

X-RAY DIFFRACTION (2.45 Å)