6F3H

Crystal structure of Dss1 exoribonuclease active site mutant D477N from Candida glabrata

6F3H の概要

• エントリーDOI: 10.2210/pdb6f3h/pdb
• 分子名称: Exoribonuclease II, mitochondrial, RNA (5'-R(P*AP*GP*AP*UP*AP*C)-3'), RNA (5'-R(P*CP*AP*CP*UP*GP*A)-3'), ... (5 entities in total)
• 機能のキーワード: 3' to 5' exoribonuclease rna degradation mitochondria, hydrolase
• 由来する生物種: Candida glabrata (Yeast); 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 194510.50

構造登録者

Razew, M.,Nowak, E.,Nowotny, M. (登録日: 2017-11-28, 公開日: 2018-01-17, 最終更新日: 2024-11-20)

主引用文献

Razew, M.,Warkocki, Z.,Taube, M.,Kolondra, A.,Czarnocki-Cieciura, M.,Nowak, E.,Labedzka-Dmoch, K.,Kawinska, A.,Piatkowski, J.,Golik, P.,Kozak, M.,Dziembowski, A.,Nowotny, M.
Structural analysis of mtEXO mitochondrial RNA degradosome reveals tight coupling of nuclease and helicase components.
Nat Commun, 9:97-97, 2018

PubMed Abstract: Nuclease and helicase activities play pivotal roles in various aspects of RNA processing and degradation. These two activities are often present in multi-subunit complexes from nucleic acid metabolism. In the mitochondrial exoribonuclease complex (mtEXO) both enzymatic activities are tightly coupled making it an excellent minimal system to study helicase-exoribonuclease coordination. mtEXO is composed of Dss1 3'-to-5' exoribonuclease and Suv3 helicase. It is the master regulator of mitochondrial gene expression in yeast. Here, we present the structure of mtEXO and a description of its mechanism of action. The crystal structure of Dss1 reveals domains that are responsible for interactions with Suv3. Importantly, these interactions are compatible with the conformational changes of Suv3 domains during the helicase cycle. We demonstrate that mtEXO is an intimate complex which forms an RNA-binding channel spanning its entire structure, with Suv3 helicase feeding the 3' end of the RNA toward the active site of Dss1.

PubMed: 29311576
DOI: 10.1038/s41467-017-02570-5

実験手法

X-RAY DIFFRACTION (2.703 Å)