6F2R

A heterotetramer of human HspB2 and HspB3

6F2R の概要

• エントリーDOI: 10.2210/pdb6f2r/pdb
• 分子名称: HspB2,Heat shock protein beta-2,Heat shock protein beta-2,Heat shock protein beta-2,Heat shock protein beta-2,Heat shock protein beta-2, Heat shock protein beta-2, Heat shock protein beta-3,Heat shock protein beta-3,Heat shock protein beta-3,Heat shock protein beta-3,Heat shock protein beta-2, ... (9 entities in total)
• 機能のキーワード: shsp, crystallin, chaperone
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 21
• 化学式量合計: 256613.85

構造登録者

Clark, A.R.,Cole, A.R.,Boelens, W.C.,Keep, N.H.,Slingsby, C. (登録日: 2017-11-27, 公開日: 2018-07-25, 最終更新日: 2024-01-17)

主引用文献

Clark, A.R.,Vree Egberts, W.,Kondrat, F.D.L.,Hilton, G.R.,Ray, N.J.,Cole, A.R.,Carver, J.A.,Benesch, J.L.P.,Keep, N.H.,Boelens, W.C.,Slingsby, C.
Terminal Regions Confer Plasticity to the Tetrameric Assembly of Human HspB2 and HspB3.
J.Mol.Biol., 430:3297-3310, 2018

PubMed Abstract: Heterogeneity in small heat shock proteins (sHsps) spans multiple spatiotemporal regimes-from fast fluctuations of part of the protein, to conformational variability of tertiary structure, plasticity of the interfaces, and polydispersity of the inter-converting, and co-assembling oligomers. This heterogeneity and dynamic nature of sHsps has significantly hindered their structural characterization. Atomic coordinates are particularly lacking for vertebrate sHsps, where most available structures are of extensively truncated homomers. sHsps play important roles in maintaining protein levels in the cell and therefore in organismal health and disease. HspB2 and HspB3 are vertebrate sHsps that are found co-assembled in neuromuscular cells, and variants thereof are associated with disease. Here, we present the structure of human HspB2/B3, which crystallized as a hetero-tetramer in a 3:1 ratio. In the HspB2/B3 tetramer, the four α-crystallin domains (ACDs) assemble into a flattened tetrahedron which is pierced by two non-intersecting approximate dyads. Assembly is mediated by flexible "nuts and bolts" involving IXI/V motifs from terminal regions filling ACD pockets. Parts of the N-terminal region bind in an unfolded conformation into the anti-parallel shared ACD dimer grooves. Tracts of the terminal regions are not resolved, most likely due to their disorder in the crystal lattice. This first structure of a full-length human sHsp heteromer reveals the heterogeneous interactions of the terminal regions and suggests a plasticity that is important for the cytoprotective functions of sHsps.

PubMed: 29969581
DOI: 10.1016/j.jmb.2018.06.047

実験手法

X-RAY DIFFRACTION (3.9 Å)