6F2C

Methylglyoxal synthase MgsA from Bacillus subtilis

6F2C の概要

• エントリーDOI: 10.2210/pdb6f2c/pdb
• 分子名称: Methylglyoxal synthase, GLYCEROL, CHLORIDE ION, ... (4 entities in total)
• 機能のキーワード: methylglyoxal synthase, protein binding, lyase
• 由来する生物種: Bacillus subtilis (strain 168)
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 212426.01

構造登録者

Dickmanns, A.,Neumann, P.,Ficner, R. (登録日: 2017-11-24, 公開日: 2018-03-07, 最終更新日: 2024-01-17)

主引用文献

Dickmanns, A.,Zschiedrich, C.P.,Arens, J.,Parfentev, I.,Gundlach, J.,Hofele, R.,Neumann, P.,Urlaub, H.,Gorke, B.,Ficner, R.,Stulke, J.
Structural basis for the regulatory interaction of the methylglyoxal synthase MgsA with the carbon flux regulator Crh inBacillus subtilis.
J. Biol. Chem., 293:5781-5792, 2018

PubMed Abstract: Utilization of energy-rich carbon sources such as glucose is fundamental to the evolutionary success of bacteria. Glucose can be catabolized via glycolysis for feeding the intermediary metabolism. The methylglyoxal synthase MgsA produces methylglyoxal from the glycolytic intermediate dihydroxyacetone phosphate. Methylglyoxal is toxic, requiring stringent regulation of MgsA activity. In the Gram-positive bacterium , an interaction with the phosphoprotein Crh controls MgsA activity. In the absence of preferred carbon sources, Crh is present in the nonphosphorylated state and binds to and thereby inhibits MgsA. To better understand the mechanism of regulation of MgsA, here we performed biochemical and structural analyses of MgsA and of its interaction with Crh. Our results indicated that MgsA forms a hexamer ( a trimer of dimers) in the crystal structure, whereas it seems to exist in an equilibrium between a dimer and hexamer in solution. In the hexamer, two alternative dimers could be distinguished, but only one appeared to prevail in solution. Further analysis strongly suggested that the hexamer is the biologically active form. cross-linking studies revealed that Crh interacts with the N-terminal helices of MgsA and that the Crh-MgsA binding inactivates MgsA by distorting and thereby blocking its active site. In summary, our results indicate that dimeric and hexameric MgsA species exist in an equilibrium in solution, that the hexameric species is the active form, and that binding to Crh deforms and blocks the active site in MgsA.

PubMed: 29514981
DOI: 10.1074/jbc.RA117.001289

実験手法

X-RAY DIFFRACTION (2.34 Å)