6EYU

Crystal structure of the inward H(+) pump xenorhodopsin

6EYU の概要

• エントリーDOI: 10.2210/pdb6eyu/pdb
• 分子名称: Bacteriorhodopsin, RETINAL, EICOSANE, ... (5 entities in total)
• 機能のキーワード: retinal protein, proton transport, membrane protein
• 由来する生物種: Nanosalina sp. (strain J07AB43)
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 88515.04

構造登録者

Kovalev, K.,Shevchenko, V.,Polovinkin, V.,Mager, T.,Gushchin, I.,Melnikov, I.,Borshchevskiy, V.,Popov, A.,Alekseev, A.,Gordeliy, V. (登録日: 2017-11-13, 公開日: 2017-12-13, 最終更新日: 2024-10-16)

主引用文献

Shevchenko, V.,Mager, T.,Kovalev, K.,Polovinkin, V.,Alekseev, A.,Juettner, J.,Chizhov, I.,Bamann, C.,Vavourakis, C.,Ghai, R.,Gushchin, I.,Borshchevskiy, V.,Rogachev, A.,Melnikov, I.,Popov, A.,Balandin, T.,Rodriguez-Valera, F.,Manstein, D.J.,Bueldt, G.,Bamberg, E.,Gordeliy, V.
Inward H(+) pump xenorhodopsin: Mechanism and alternative optogenetic approach.
Sci Adv, 3:e1603187-, 2017

PubMed Abstract: Generation of an electrochemical proton gradient is the first step of cell bioenergetics. In prokaryotes, the gradient is created by outward membrane protein proton pumps. Inward plasma membrane native proton pumps are yet unknown. We describe comprehensive functional studies of the representatives of the yet noncharacterized xenorhodopsins from Nanohaloarchaea family of microbial rhodopsins. They are inward proton pumps as we demonstrate in model membrane systems, cells, human embryonic kidney cells, neuroblastoma cells, and rat hippocampal neuronal cells. We also solved the structure of a xenorhodopsin from the nanohalosarchaeon (XeR) and suggest a mechanism of inward proton pumping. We demonstrate that the XeR is a powerful pump, which is able to elicit action potentials in rat hippocampal neuronal cells up to their maximal intrinsic firing frequency. Hence, inwardly directed proton pumps are suitable for light-induced remote control of neurons, and they are an alternative to the well-known cation-selective channelrhodopsins.

PubMed: 28948217
DOI: 10.1126/sciadv.1603187

実験手法

X-RAY DIFFRACTION (2.5 Å)