6EXZ

Crystal structure of Mex67 C-term

Summary for 6EXZ

• Entry DOI: 10.2210/pdb6exz/pdb
• Descriptor: mRNA export factor MEX67, FORMIC ACID (3 entities in total)
• Functional Keywords: mrna export, transport protein
• Biological source: Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
• Total number of polymer chains: 1
• Total formula weight: 8333.55

Authors

Mohamad, N.,Bravo, J. (deposition date: 2017-11-10, release date: 2018-11-21, Last modification date: 2024-01-17)

Primary citation

Martin-Exposito, M.,Gas, M.E.,Mohamad, N.,Nuno-Cabanes, C.,Tejada-Colon, A.,Pascual-Garcia, P.,de la Fuente, L.,Chaves-Arquero, B.,Merran, J.,Corden, J.,Conesa, A.,Perez-Canadillas, J.M.,Bravo, J.,Rodriguez-Navarro, S.
Mip6 binds directly to the Mex67 UBA domain to maintain low levels of Msn2/4 stress-dependent mRNAs.
Embo Rep., :e47964-e47964, 2019

PubMed Abstract: RNA-binding proteins (RBPs) participate in all steps of gene expression, underscoring their potential as regulators of RNA homeostasis. We structurally and functionally characterize Mip6, a four-RNA recognition motif (RRM)-containing RBP, as a functional and physical interactor of the export factor Mex67. Mip6-RRM4 directly interacts with the ubiquitin-associated (UBA) domain of Mex67 through a loop containing tryptophan 442. Mip6 shuttles between the nucleus and the cytoplasm in a Mex67-dependent manner and concentrates in cytoplasmic foci under stress. Photoactivatable ribonucleoside-enhanced crosslinking and immunoprecipitation experiments show preferential binding of Mip6 to mRNAs regulated by the stress-response Msn2/4 transcription factors. Consistent with this binding, MIP6 deletion affects their export and expression levels. Additionally, Mip6 interacts physically and/or functionally with proteins with a role in mRNA metabolism and transcription such as Rrp6, Xrn1, Sgf73, and Rpb1. These results reveal a novel role for Mip6 in the homeostasis of Msn2/4-dependent transcripts through its direct interaction with the Mex67 UBA domain.

PubMed: 31680439
DOI: 10.15252/embr.201947964

Experimental method

X-RAY DIFFRACTION (1.3 Å)