6EVU

Adhesin domain of PrgB from Enterococcus faecalis

6EVU の概要

• エントリーDOI: 10.2210/pdb6evu/pdb
• 分子名称: PrgB, PHOSPHATE ION, 2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• 機能のキーワード: adhesin, cell adhesion, biofilm formation
• 由来する生物種: Enterococcus faecalis (Streptococcus faecalis)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 34378.14

構造登録者

Schmitt, A.,Berntsson, R.P.A. (登録日: 2017-11-02, 公開日: 2018-05-16, 最終更新日: 2024-05-08)

主引用文献

Schmitt, A.,Jiang, K.,Camacho, M.I.,Jonna, V.R.,Hofer, A.,Westerlund, F.,Christie, P.J.,Berntsson, R.P.
PrgB promotes aggregation, biofilm formation, and conjugation through DNA binding and compaction.
Mol. Microbiol., 109:291-305, 2018

PubMed Abstract: Gram-positive bacteria deploy type IV secretion systems (T4SSs) to facilitate horizontal gene transfer. The T4SSs of Gram-positive bacteria rely on surface adhesins as opposed to conjugative pili to facilitate mating. Enterococcus faecalis PrgB is a surface adhesin that promotes mating pair formation and robust biofilm development in an extracellular DNA (eDNA) dependent manner. Here, we report the structure of the adhesin domain of PrgB. The adhesin domain binds and compacts DNA in vitro. In vivo PrgB deleted of its adhesin domain does not support cellular aggregation, biofilm development and conjugative DNA transfer. PrgB also binds lipoteichoic acid (LTA), which competes with DNA binding. We propose that PrgB binding and compaction of eDNA facilitates cell aggregation and plays an important role in establishment of early biofilms in mono- or polyspecies settings. Within these biofilms, PrgB mediates formation and stabilization of direct cell-cell contacts through alternative binding of cell-bound LTA, which in turn promotes establishment of productive mating junctions and efficient intra- or inter-species T4SS-mediated gene transfer.

PubMed: 29723434
DOI: 10.1111/mmi.13980

実験手法

X-RAY DIFFRACTION (1.598 Å)