6EVG

Structural and Functional Characterisation of a Bacterial Laccase-like Multi-copper Oxidase CueO from Lignin-Degrading Bacterium Ochrobactrum sp. with Oxidase Activity towards Lignin Model Compounds and Lignosulfonate

6EVG の概要

• エントリーDOI: 10.2210/pdb6evg/pdb
• 分子名称: Multi-copper Oxidase CueO, COPPER (II) ION, 1,2-ETHANEDIOL, ... (4 entities in total)
• 機能のキーワード: laccase, ochrobactrum, lignin degradation, copper protein, oxidoreductase
• 由来する生物種: Ochrobactrum
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 55322.94

構造登録者

Fulop, V.,Wilkinson, R. (登録日: 2017-11-01, 公開日: 2018-04-11, 最終更新日: 2024-01-17)

主引用文献

Granja-Travez, R.S.,Wilkinson, R.C.,Persinoti, G.F.,Squina, F.M.,Fulop, V.,Bugg, T.D.H.
Structural and functional characterisation of multi-copper oxidase CueO from lignin-degrading bacterium Ochrobactrum sp. reveal its activity towards lignin model compounds and lignosulfonate.
FEBS J., 285:1684-1700, 2018

PubMed Abstract: The identification of enzymes responsible for oxidation of lignin in lignin-degrading bacteria is of interest for biotechnological valorization of lignin to renewable chemical products. The genome sequences of two lignin-degrading bacteria, Ochrobactrum sp., and Paenibacillus sp., contain no B-type DyP peroxidases implicated in lignin degradation in other bacteria, but contain putative multicopper oxidase genes. Multi-copper oxidase CueO from Ochrobactrum sp. was expressed and reconstituted as a recombinant laccase-like enzyme, and kinetically characterized. Ochrobactrum CueO shows activity for oxidation of β-aryl ether and biphenyl lignin dimer model compounds, generating oxidized dimeric products, and shows activity for oxidation of Ca-lignosulfonate, generating vanillic acid as a low molecular weight product. The crystal structure of Ochrobactrum CueO (OcCueO) has been determined at 1.1 Å resolution (PDB: 6EVG), showing a four-coordinate mononuclear type I copper center with ligands His495, His434 and Cys490 with Met500 as an axial ligand, similar to that of Escherichia coli CueO and bacterial azurin proteins, whereas fungal laccase enzymes contain a three-coordinate type I copper metal center. A trinuclear type 2/3 copper cluster was modeled into the active site, showing similar structure to E. coli CueO and fungal laccases, and three solvent channels leading to the active site. Site-directed mutagenesis was carried out on amino acid residues found in the solvent channels, indicating the importance for residues Asp102, Gly103, Arg221, Arg223, and Asp462 for catalytic activity. The work identifies a new bacterial multicopper enzyme with activity for lignin oxidation, and implicates a role for bacterial laccase-like multicopper oxidases in some lignin-degrading bacteria.

PubMed: 29575798
DOI: 10.1111/febs.14437

実験手法

X-RAY DIFFRACTION (1.1 Å)