6EU9

Crystal structure of Platynereis dumerilii RAR ligand-binding domain in complex with all-trans retinoic acid

6EU9 の概要

• エントリーDOI: 10.2210/pdb6eu9/pdb
• 分子名称: Retinoic acid receptor, RETINOIC ACID (3 entities in total)
• 機能のキーワード: retinoic acid, ancestral receptor, sensor, transcription
• 由来する生物種: Platynereis dumerilii
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 110434.63

構造登録者

Handberg-Thorsager, M.,Gutierrez-Mazariegos, J.,Arold, S.T.,Nadendla, E.K.,Bertucci, P.Y.,Germain, P.,Tomancak, P.,Pierzchalski, K.,Jones, J.W.,Albalat, R.,Kane, M.A.,Bourguet, W.,Laudet, V.,Arendt, D.,Schubert, M. (登録日: 2017-10-29, 公開日: 2018-03-14, 最終更新日: 2024-05-08)

主引用文献

Handberg-Thorsager, M.,Gutierrez-Mazariegos, J.,Arold, S.T.,Kumar Nadendla, E.,Bertucci, P.Y.,Germain, P.,Tomancak, P.,Pierzchalski, K.,Jones, J.W.,Albalat, R.,Kane, M.A.,Bourguet, W.,Laudet, V.,Arendt, D.,Schubert, M.
The ancestral retinoic acid receptor was a low-affinity sensor triggering neuronal differentiation.
Sci Adv, 4:eaao1261-eaao1261, 2018

PubMed Abstract: Retinoic acid (RA) is an important intercellular signaling molecule in vertebrate development, with a well-established role in the regulation of genes during hindbrain patterning and in neurogenesis. However, the evolutionary origin of the RA signaling pathway remains elusive. To elucidate the evolution of the RA signaling system, we characterized RA metabolism and signaling in the marine annelid , a powerful model for evolution, development, and neurobiology. Binding assays and crystal structure analyses show that the annelid retinoic acid receptor (RAR) binds RA and activates transcription just as vertebrate RARs, yet with a different ligand-binding pocket and lower binding affinity, suggesting a permissive rather than instructive role of RA signaling. RAR knockdown and RA treatment of swimming annelid larvae further reveal that the RA signal is locally received in the medial neuroectoderm, where it controls neurogenesis and axon outgrowth, whereas the spatial colinear gene expression in the neuroectoderm remains unaffected. These findings suggest that one early role of the new RAR in bilaterian evolution was to control the spatially restricted onset of motor and interneuron differentiation in the developing ventral nerve cord and to indicate that the regulation of -controlled anterior-posterior patterning arose only at the base of the chordates, concomitant with a high-affinity RAR needed for the interpretation of a complex RA gradient.

PubMed: 29492455
DOI: 10.1126/sciadv.aao1261

実験手法

X-RAY DIFFRACTION (2.69 Å)