6ETL
Atomic resolution structure of RNase A (data collection 2)
Summary for 6ETL
| Entry DOI | 10.2210/pdb6etl/pdb |
| Descriptor | Ribonuclease pancreatic, ISOPROPYL ALCOHOL (3 entities in total) |
| Functional Keywords | raman microspectroscopy, ribonuclease, atomic resolution, radiation damage, photodamage, hydrolase |
| Biological source | Bos taurus (Bovine) |
| Cellular location | Secreted: P61823 |
| Total number of polymer chains | 1 |
| Total formula weight | 13888.61 |
| Authors | Caterino, M.,Vergara, A.,Merlino, A. (deposition date: 2017-10-27, release date: 2018-02-21, Last modification date: 2024-11-20) |
| Primary citation | Vergara, A.,Caterino, M.,Merlino, A. Raman-markers of X-ray radiation damage of proteins. Int. J. Biol. Macromol., 111:1194-1205, 2018 Cited by PubMed Abstract: Despite their high relevance, the mechanisms of X-ray radiation damage on protein structure yet have to be completely established. Here, we used Raman microspectrophotometry to follow X-ray-induced chemical modifications on the structure of the model protein bovine pancreatic ribonuclease (RNase A). The combination of dose-dependent Raman spectra and ultrahigh resolution (eight structures solved using data collected between 0.85 and 1.17 Å resolution on the same single crystal) allowed direct observation of several radiation damage events, including covalent bond breakages and formation of radicals. Our results are relevant for analytical photodamage detection and provide implications for a detailed understanding of the mechanisms of photoproduct formation. PubMed: 29374529DOI: 10.1016/j.ijbiomac.2018.01.135 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (0.85 Å) |
Structure validation
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