6ESB

BK polyomavirus + 20 mM GT1b oligosaccharide

6ESB の概要

• エントリーDOI: 10.2210/pdb6esb/pdb
• EMDBエントリー: 3944
• 分子名称: Capsid protein VP1, Minor capsid protein VP2, N-acetyl-alpha-neuraminic acid-(2-8)-N-acetyl-alpha-neuraminic acid, ... (4 entities in total)
• 機能のキーワード: polyomavirus, receptor, complex, glycan, virus
• 由来する生物種: BK polyomavirus (BKPyV); 詳細
• タンパク質・核酸の鎖数: 7
• 化学式量合計: 282417.04

構造登録者

Hurdiss, D.L.,Ranson, N.A. (登録日: 2017-10-20, 公開日: 2018-05-02, 最終更新日: 2024-05-15)

主引用文献

Hurdiss, D.L.,Frank, M.,Snowden, J.S.,Macdonald, A.,Ranson, N.A.
The Structure of an Infectious Human Polyomavirus and Its Interactions with Cellular Receptors.
Structure, 26:839-847.e3, 2018

PubMed Abstract: BK polyomavirus (BKV) causes polyomavirus-associated nephropathy and hemorrhagic cystitis in immunosuppressed patients. These are diseases for which we currently have limited treatment options, but potential therapies could include pre-transplant vaccination with a multivalent BKV vaccine or therapeutics which inhibit capsid assembly or block attachment and entry into target cells. A useful tool in such efforts would be a high-resolution structure of the infectious BKV virion and how this interacts with its full repertoire of cellular receptors. We present the 3.4-Å cryoelectron microscopy structure of native, infectious BKV in complex with the receptor fragment of GT1b ganglioside. We also present structural evidence that BKV can utilize glycosaminoglycans as attachment receptors. This work highlights features that underpin capsid stability and provides a platform for rational design and development of urgently needed pharmacological interventions for BKV-associated diseases.

PubMed: 29706532
DOI: 10.1016/j.str.2018.03.019

実験手法

ELECTRON MICROSCOPY (3.4 Å)