6ES8

HIV capsid hexamer with IP6 ligand

6ES8 の概要

• エントリーDOI: 10.2210/pdb6es8/pdb
• 分子名称: Gag protein, INOSITOL HEXAKISPHOSPHATE (3 entities in total)
• 機能のキーワード: hiv, viral protein
• 由来する生物種: Human immunodeficiency virus 1
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25164.21

構造登録者

James, L.C. (登録日: 2017-10-19, 公開日: 2018-08-15, 最終更新日: 2025-10-01)

主引用文献

Mallery, D.L.,Marquez, C.L.,McEwan, W.A.,Dickson, C.F.,Jacques, D.A.,Anandapadamanaban, M.,Bichel, K.,Towers, G.J.,Saiardi, A.,Bocking, T.,James, L.C.
IP6 is an HIV pocket factor that prevents capsid collapse and promotes DNA synthesis.
Elife, 7:-, 2018

PubMed Abstract: The HIV capsid is semipermeable and covered in electropositive pores that are essential for viral DNA synthesis and infection. Here, we show that these pores bind the abundant cellular polyanion IP, transforming viral stability from minutes to hours and allowing newly synthesised DNA to accumulate inside the capsid. An arginine ring within the pore coordinates IP, which strengthens capsid hexamers by almost 10°C. Single molecule measurements demonstrate that this renders native HIV capsids highly stable and protected from spontaneous collapse. Moreover, encapsidated reverse transcription assays reveal that, once stabilised by IP, the accumulation of new viral DNA inside the capsid increases >100 fold. Remarkably, isotopic labelling of inositol in virus-producing cells reveals that HIV selectively packages over 300 IP molecules per infectious virion. We propose that HIV recruits IP to regulate capsid stability and uncoating, analogous to picornavirus pocket factors. HIV-1/IP/capsid/co-factor/reverse transcription.

PubMed: 29848441
DOI: 10.7554/eLife.35335

実験手法

X-RAY DIFFRACTION (1.9 Å)