6EOK

Crystal structure of E. coli L-asparaginase II

6EOK の概要

• エントリーDOI: 10.2210/pdb6eok/pdb
• 関連するPDBエントリー: 1IHD 1JAZ 1NNS 3ECA
• 分子名称: L-asparaginase 2, ZINC ION (3 entities in total)
• 機能のキーワード: l-asparaginase ii, hydrolase
• 由来する生物種: Escherichia coli (strain K12)
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 138768.87

構造登録者

Cerofolini, L.,Giuntini, S.,Carlon, A.,Ravera, E.,Calderone, V.,Fragai, M.,Parigi, G.,Luchinat, C. (登録日: 2017-10-09, 公開日: 2018-10-31, 最終更新日: 2024-11-06)

主引用文献

Cerofolini, L.,Giuntini, S.,Carlon, A.,Ravera, E.,Calderone, V.,Fragai, M.,Parigi, G.,Luchinat, C.
Characterization of PEGylated Asparaginase: New Opportunities from NMR Analysis of Large PEGylated Therapeutics.
Chemistry, 25:1984-1991, 2019

PubMed Abstract: Resonance assignment and structural characterization of pharmacologically relevant proteins promise to improve understanding and safety of these proteins by rational design. However, the PEG coating that is used to evade the immune system also causes these molecules to "evade" the standard structural biology methodologies. We here demonstrate that it is possible to obtain the resonance assignment and a reliable structural model of large PEGylated proteins through an integrated approach encompassing NMR and X-ray crystallography.

PubMed: 30462348
DOI: 10.1002/chem.201804488

実験手法

X-RAY DIFFRACTION (2.5 Å)