6ELK

C.elegans MnSOD-3 mutant - Q142H

6ELK の概要

• エントリーDOI: 10.2210/pdb6elk/pdb
• 関連するPDBエントリー: 3DC5 4X9Q
• 分子名称: Superoxide dismutase [Mn] 2, mitochondrial, MANGANESE (II) ION, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: superoxide dismutase, manganese, oxidoreductase
• 由来する生物種: Caenorhabditis elegans
• 細胞内の位置: Mitochondrion : P41977
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 45517.34

構造登録者

Hunter, G.J.,Trinh, C.H.,Hunter, T. (登録日: 2017-09-29, 公開日: 2017-12-06, 最終更新日: 2024-01-17)

主引用文献

Hunter, T.,Bonetta, R.,Sacco, A.,Vella, M.,Sultana, P.M.,Trinh, C.H.,Fadia, H.B.R.,Borowski, T.,Garcia-Fandino, R.,Stockner, T.,Hunter, G.J.
A Single Mutation is Sufficient to Modify the Metal Selectivity and Specificity of a Eukaryotic Manganese Superoxide Dismutase to Encompass Iron.
Chemistry, 24:5303-5308, 2018

PubMed Abstract: We have generated a site-directed mutant of the manganese superoxide dismutase SOD-3 of C.elegans (MnSOD-3) which modifies the metal specificity of the enzyme. While wild-type MnSOD-3 functions with manganese in the active site (3600 U mg of protein) it has little or no activity when iron is incorporated. However, when histidine replaces glutamine 142 in the active site, the enzyme retains 50 % of its activity and becomes cambialistic for its metal cofactor exhibiting very similar specific activity with either manganese or iron.

PubMed: 29178484
DOI: 10.1002/chem.201704655

実験手法

X-RAY DIFFRACTION (1.65 Å)