6EKT

Structure of P47 from Clostridium botulinum A2

6EKT の概要

• エントリーDOI: 10.2210/pdb6ekt/pdb
• 分子名称: p-47 protein (2 entities in total)
• 機能のキーワード: lipid binding, lipid binding protein
• 由来する生物種: Clostridium botulinum (strain Kyoto / Type A2)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 50073.55

構造登録者

Berntsson, R.P.,Stenmark, P. (登録日: 2017-09-27, 公開日: 2017-10-25, 最終更新日: 2024-05-08)

主引用文献

Gustafsson, R.,Berntsson, R.P.,Martinez-Carranza, M.,El Tekle, G.,Odegrip, R.,Johnson, E.A.,Stenmark, P.
Crystal structures of OrfX2 and P47 from a Botulinum neurotoxin OrfX-type gene cluster.
FEBS Lett., 591:3781-3792, 2017

PubMed Abstract: Botulinum neurotoxins are highly toxic substances and are all encoded together with one of two alternative gene clusters, the HA or the OrfX gene cluster. Very little is known about the function and structure of the proteins encoded in the OrfX gene cluster, which in addition to the toxin contains five proteins (OrfX1, OrfX2, OrfX3, P47, and NTNH). We here present the structures of OrfX2 and P47, solved to 2.1 and 1.8 Å, respectively. We show that they belong to the TULIP protein superfamily, which are often involved in lipid binding. OrfX1 and OrfX2 were both found to bind phosphatidylinositol lipids.

PubMed: 29067689
DOI: 10.1002/1873-3468.12889

実験手法

X-RAY DIFFRACTION (1.75 Å)