6EIZ

A de novo designed hexameric coiled coil CC-Hex2 with farnesol bound in the channel.

6EIZ の概要

• エントリーDOI: 10.2210/pdb6eiz/pdb
• 関連するPDBエントリー: 6EIK
• 分子名称: CC-Hex2, (2E,6E)-3,7,11-trimethyldodeca-2,6,10-trien-1-ol (3 entities in total)
• 機能のキーワード: alpha-helical coiled-coil barrel, de novo protein
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 20028.20

構造登録者

Rhys, G.G.,Burton, A.J.,Dawson, W.M.,Thomas, F.,Woolfson, D.N. (登録日: 2017-09-19, 公開日: 2018-07-11, 最終更新日: 2024-11-20)

主引用文献

Thomas, F.,Dawson, W.M.,Lang, E.J.M.,Burton, A.J.,Bartlett, G.J.,Rhys, G.G.,Mulholland, A.J.,Woolfson, D.N.
De Novo-Designed alpha-Helical Barrels as Receptors for Small Molecules.
ACS Synth Biol, 7:1808-1816, 2018

PubMed Abstract: We describe de novo-designed α-helical barrels (αHBs) that bind and discriminate between lipophilic biologically active molecules. αHBs have five or more α-helices arranged around central hydrophobic channels the diameters of which scale with oligomer state. We show that pentameric, hexameric, and heptameric αHBs bind the environmentally sensitive dye 1,6-diphenylhexatriene (DPH) in the micromolar range and fluoresce. Displacement of the dye is used to report the binding of nonfluorescent molecules: palmitic acid and retinol bind to all three αHBs with submicromolar inhibitor constants; farnesol binds the hexamer and heptamer; but β-carotene binds only the heptamer. A co-crystal structure of the hexamer with farnesol reveals oriented binding in the center of the hydrophobic channel. Charged side chains engineered into the lumen of the heptamer facilitate binding of polar ligands: a glutamate variant binds a cationic variant of DPH, and introducing lysine allows binding of the biosynthetically important farnesol diphosphate.

PubMed: 29944338
DOI: 10.1021/acssynbio.8b00225

実験手法

X-RAY DIFFRACTION (1.85 Å)