6EIT

Coxsackievirus A24v in complex with the D1-D2 fragment of ICAM-1

Summary for 6EIT

• Entry DOI: 10.2210/pdb6eit/pdb
• EMDB information: 3880
• Descriptor: VP1, VP2, VP3, ... (4 entities in total)
• Functional Keywords: enterovirus, receptor, complex, picornavirus, virus
• Biological source: Homo sapiens (Human); More
• Cellular location: Virion : G3C8J7; Host cytoplasmic vesicle membrane ; Peripheral membrane protein ; Cytoplasmic side : A0A088F913 Q0GYP7; Membrane; Single-pass type I membrane protein: P05362
• Total number of polymer chains: 4
• Total formula weight: 100131.13

Authors

Hurdiss, D.L.,Ranson, N.A. (deposition date: 2017-09-19, release date: 2018-01-10, Last modification date: 2024-11-13)

Primary citation

Baggen, J.,Hurdiss, D.L.,Zocher, G.,Mistry, N.,Roberts, R.W.,Slager, J.J.,Guo, H.,van Vliet, A.L.W.,Wahedi, M.,Benschop, K.,Duizer, E.,de Haan, C.A.M.,de Vries, E.,Casasnovas, J.M.,de Groot, R.J.,Arnberg, N.,Stehle, T.,Ranson, N.A.,Thibaut, H.J.,van Kuppeveld, F.J.M.
Role of enhanced receptor engagement in the evolution of a pandemic acute hemorrhagic conjunctivitis virus.
Proc. Natl. Acad. Sci. U.S.A., 115:397-402, 2018

PubMed Abstract: Acute hemorrhagic conjunctivitis (AHC) is a painful, contagious eye disease, with millions of cases in the last decades. Coxsackievirus A24 (CV-A24) was not originally associated with human disease, but in 1970 a pathogenic "variant" (CV-A24v) emerged, which is now the main cause of AHC. Initially, this variant circulated only in Southeast Asia, but it later spread worldwide, accounting for numerous AHC outbreaks and two pandemics. While both CV-A24 variant and nonvariant strains still circulate in humans, only variant strains cause AHC for reasons that are yet unknown. Since receptors are important determinants of viral tropism, we set out to map the CV-A24 receptor repertoire and establish whether changes in receptor preference have led to the increased pathogenicity and rapid spread of CV-A24v. Here, we identify ICAM-1 as an essential receptor for both AHC-causing and non-AHC strains. We provide a high-resolution cryo-EM structure of a virus-ICAM-1 complex, which revealed critical ICAM-1-binding residues. These data could help identify a possible conserved mode of receptor engagement among ICAM-1-binding enteroviruses and rhinoviruses. Moreover, we identify a single capsid substitution that has been adopted by all pandemic CV-A24v strains and we reveal that this adaptation enhances the capacity of CV-A24v to bind sialic acid. Our data elucidate the CV-A24v receptor repertoire and point to a role of enhanced receptor engagement in the adaptation to the eye, possibly enabling pandemic spread.

PubMed: 29284752
DOI: 10.1073/pnas.1713284115

Experimental method

ELECTRON MICROSCOPY (3.9 Å)