6EI6

CC2D1B coordinates ESRCT-III activity during the mitotic reformation of the nuclear envelope

6EI6 の概要

• エントリーDOI: 10.2210/pdb6ei6/pdb
• 分子名称: Coiled-coil and C2 domain-containing protein 1-like, SULFATE ION, DI(HYDROXYETHYL)ETHER, ... (4 entities in total)
• 機能のキーワード: escrt protein regulator, nuclear envelope reformation, polymerization, cytosolic protein
• 由来する生物種: Drosophila melanogaster (Fruit fly)
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 63262.00

構造登録者

Ventimiglia, L.N.,Cuesta-Geijo, M.A.,Martinelli, N.,Caballe, A.,Macheboeuf, P.,Miguet, N.,Parnham, I.M.,Olmos, Y.,Carlton, J.G.,Weissehorn, W.,martin-Serrano, J. (登録日: 2017-09-18, 公開日: 2018-10-10, 最終更新日: 2024-10-16)

主引用文献

Ventimiglia, L.N.,Cuesta-Geijo, M.A.,Martinelli, N.,Caballe, A.,Macheboeuf, P.,Miguet, N.,Parnham, I.M.,Olmos, Y.,Carlton, J.G.,Weissenhorn, W.,Martin-Serrano, J.
CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.
Dev. Cell, 47:547-563.e6, 2018

PubMed Abstract: The coordinated reformation of the nuclear envelope (NE) after mitosis re-establishes the structural integrity and the functionality of the nuclear compartment. The endosomal sorting complex required for transport (ESCRT) machinery, a membrane remodeling pathway that is highly conserved in eukaryotes, has been recently involved in NE resealing by mediating the annular fusion of the nuclear membrane (NM). We show here that CC2D1B, a regulator of ESCRT polymerization, is required to re-establish the nuclear compartmentalization by coordinating endoplasmic reticulum (ER) membrane deposition around chromatin disks with ESCRT-III recruitment to the reforming NE. Accordingly, CC2D1B determines the spatiotemporal distribution of the CHMP7-ESCRT-III axis during NE reformation. Crucially, in CC2D1B-depleted cells, ESCRT activity is uncoupled from Spastin-mediated severing of spindle microtubules, resulting in persisting microtubules that compromise nuclear morphology. Therefore, we reveal CC2D1B as an essential regulatory factor that licenses the formation of ESCRT-III polymers to ensure the orderly reformation of the NE.

PubMed: 30513301
DOI: 10.1016/j.devcel.2018.11.012

実験手法

X-RAY DIFFRACTION (2.461 Å)