6EDK

Crystal structure of the formyltransferase PseJ from Anoxybacillus kamchatkensis with N10-formyltetrahydrofolate

6EDK の概要

• エントリーDOI: 10.2210/pdb6edk/pdb
• 分子名称: Formyltransferase PseJ, 2-(N-MORPHOLINO)-ETHANESULFONIC ACID, SULFATE ION, ... (5 entities in total)
• 機能のキーワード: formyltransferase, transferase
• 由来する生物種: Anoxybacillus kamchatkensis G10
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 26373.79

構造登録者

Reimer, J.M.,Harb, I.,Schmeing, T.M. (登録日: 2018-08-09, 公開日: 2018-10-17, 最終更新日: 2023-10-11)

主引用文献

Reimer, J.M.,Harb, I.,Ovchinnikova, O.G.,Jiang, J.,Whitfield, C.,Schmeing, T.M.
Structural Insight into a Novel Formyltransferase and Evolution to a Nonribosomal Peptide Synthetase Tailoring Domain.
ACS Chem. Biol., 13:3161-3172, 2018

PubMed Abstract: Nonribosomal peptide synthetases (NRPSs) increase the chemical diversity of their products by acquiring tailoring domains. Linear gramicidin synthetase starts with a tailoring formylation (F) domain, which likely originated from a sugar formyltransferase (FT) gene. Here, we present studies on an Anoxybacillus kamchatkensis sugar FT representative of the prehorizontal gene transfer FT. Gene cluster analysis reveals that this FT acts on a UDP-sugar in a novel pathway for synthesis of a 7-formamido derivative of CMP-pseudaminic acid. We recapitulate the pathway up to and including the formylation step in vitro, experimentally demonstrating the role of the FT. We also present X-ray crystal structures of the FT alone and with ligands, which unveil contrasts with other structurally characterized sugar FTs and show close structural similarity with the F domain. The structures reveal insights into the adaptations that were needed to co-opt and evolve a sugar FT into a functional and useful NRPS domain.

PubMed: 30346688
DOI: 10.1021/acschembio.8b00739

実験手法

X-RAY DIFFRACTION (1.8 Å)