6ECC

Vlm2 thioesterase domain wild type structure 2

6ECC の概要

• エントリーDOI: 10.2210/pdb6ecc/pdb
• 関連するPDBエントリー: 6ECB 6ECD 6ECE 6ECF
• 分子名称: Vlm2 (2 entities in total)
• 機能のキーワード: thioesterase, thioesterase domain, nrps, non-ribosomal peptide synthetase, nonribosomal peptide synthetase, valinomycin, hydrolase, depsipeptide
• 由来する生物種: Streptomyces tsusimaensis
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 33096.50

構造登録者

Alonzo, D.A.,Schmeing, T.M. (登録日: 2018-08-07, 公開日: 2018-12-12, 最終更新日: 2023-10-11)

主引用文献

Huguenin-Dezot, N.,Alonzo, D.A.,Heberlig, G.W.,Mahesh, M.,Nguyen, D.P.,Dornan, M.H.,Boddy, C.N.,Schmeing, T.M.,Chin, J.W.
Trapping biosynthetic acyl-enzyme intermediates with encoded 2,3-diaminopropionic acid.
Nature, 565:112-117, 2019

PubMed Abstract: Many enzymes catalyse reactions that proceed through covalent acyl-enzyme (ester or thioester) intermediates. These enzymes include serine hydrolases (encoded by one per cent of human genes, and including serine proteases and thioesterases), cysteine proteases (including caspases), and many components of the ubiquitination machinery. Their important acyl-enzyme intermediates are unstable, commonly having half-lives of minutes to hours. In some cases, acyl-enzyme complexes can be stabilized using substrate analogues or active-site mutations but, although these approaches can provide valuable insight, they often result in complexes that are substantially non-native. Here we develop a strategy for incorporating 2,3-diaminopropionic acid (DAP) into recombinant proteins, via expansion of the genetic code. We show that replacing catalytic cysteine or serine residues of enzymes with DAP permits their first-step reaction with native substrates, allowing the efficient capture of acyl-enzyme complexes that are linked through a stable amide bond. For one of these enzymes, the thioesterase domain of valinomycin synthetase, we elucidate the biosynthetic pathway by which it progressively oligomerizes tetradepsipeptidyl substrates to a dodecadepsipeptidyl intermediate, which it then cyclizes to produce valinomycin. By trapping the first and last acyl-thioesterase intermediates in the catalytic cycle as DAP conjugates, we provide structural insight into how conformational changes in thioesterase domains of such nonribosomal peptide synthetases control the oligomerization and cyclization of linear substrates. The encoding of DAP will facilitate the characterization of diverse acyl-enzyme complexes, and may be extended to capturing the native substrates of transiently acylated proteins of unknown function.

PubMed: 30542153
DOI: 10.1038/s41586-018-0781-z

実験手法

X-RAY DIFFRACTION (1.8 Å)