6E9D

Sub-2 Angstrom Ewald Curvature-Corrected Single-Particle Cryo-EM Reconstruction of AAV-2 L336C

これはPDB形式変換不可エントリーです。

6E9D の概要

• エントリーDOI: 10.2210/pdb6e9d/pdb
• EMDBエントリー: 9012
• 分子名称: Capsid protein VP1 (2 entities in total)
• 機能のキーワード: aav-2 l336c, gene therapy, ewald sphere curvature correction, per particle ctf, virus like particle
• 由来する生物種: Adeno-associated virus 2 Srivastava/1982 (AAV-2)
• タンパク質・核酸の鎖数: 60
• 化学式量合計: 4921280.16

構造登録者

Tan, Y.Z.,Aiyer, S.,Mietzsch, M.,Hull, J.A.,McKenna, R.,Baker, T.S.,Agbandje-McKenna, M.,Lyumkis, D. (登録日: 2018-07-31, 公開日: 2018-08-15, 最終更新日: 2024-03-13)

主引用文献

Tan, Y.Z.,Aiyer, S.,Mietzsch, M.,Hull, J.A.,McKenna, R.,Grieger, J.,Samulski, R.J.,Baker, T.S.,Agbandje-McKenna, M.,Lyumkis, D.
Sub-2 angstrom Ewald curvature corrected structure of an AAV2 capsid variant.
Nat Commun, 9:3628-3628, 2018

PubMed Abstract: Single-particle cryogenic electron microscopy (cryo-EM) provides a powerful methodology for structural biologists, but the resolutions typically attained with experimentally determined structures have lagged behind microscope capabilities. Here, we exploit several technical advances to improve resolution, including per-particle contrast transfer function (CTF) refinement and correction for Ewald sphere curvature. The latter is demonstrated with several experimental samples and should become more standard as resolutions increase or at lower microscope accelerating voltages. The combined application of the described methods to micrographs recorded on a Titan Krios enables structure determination at ~1.86-Å resolution of an adeno-associated virus serotype 2 variant (AAV2), an important gene-delivery vehicle. The resulting structural details provide an improved model for understanding the biology of AAV that will guide future vector development for gene therapy.

PubMed: 30194371
DOI: 10.1038/s41467-018-06076-6

実験手法

ELECTRON MICROSCOPY (1.86 Å)