6E7C

14-pf 3-start GMPCPP-human alpha1B/beta2B microtubules

6E7C の概要

• エントリーDOI: 10.2210/pdb6e7c/pdb
• EMDBエントリー: 8997 8998
• 分子名称: Tubulin beta-2B chain, Tubulin alpha-1B chain, MAGNESIUM ION, ... (5 entities in total)
• 機能のキーワード: cytoskeleton, microtubules, recombinant human tubulin, tubulin isotype, structural protein
• 由来する生物種: Homo sapiens (Human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 97676.00

構造登録者

Ti, S.C.,Alushin, G.M.,Kapoor, T.M. (登録日: 2018-07-25, 公開日: 2018-10-10, 最終更新日: 2024-03-13)

主引用文献

Ti, S.C.,Alushin, G.M.,Kapoor, T.M.
Human beta-Tubulin Isotypes Can Regulate Microtubule Protofilament Number and Stability.
Dev. Cell, 47:175-, 2018

PubMed Abstract: Cell biological studies have shown that protofilament number, a fundamental feature of microtubules, can correlate with the expression of different tubulin isotypes. However, it is not known if tubulin isotypes directly control this basic microtubule property. Here, we report high-resolution cryo-EM reconstructions (3.5-3.65 Å) of purified human α1B/β3 and α1B/β2B microtubules and find that the β-tubulin isotype can determine protofilament number. Comparisons of atomic models of 13- and 14-protofilament microtubules reveal how tubulin subunit plasticity, manifested in "accordion-like" distributed structural changes, can accommodate distinct lattice organizations. Furthermore, compared to α1B/β3 microtubules, α1B/β2B filaments are more stable to passive disassembly and against depolymerization by MCAK or chTOG, microtubule-associated proteins with distinct mechanisms of action. Mixing tubulin isotypes in different proportions results in microtubules with protofilament numbers and stabilities intermediate to those of isotypically pure filaments. Together, our findings indicate that microtubule protofilament number and stability can be controlled through β-tubulin isotype composition.

PubMed: 30245156
DOI: 10.1016/j.devcel.2018.08.014

実験手法

ELECTRON MICROSCOPY (3.65 Å)